A5VNA8 (RNPH_BRUO2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	BOV_0167

Organism

Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) [Complete proteome] [HAMAP]

Taxonomic identifier

444178 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP MF_00564		PRO_1000024782

Sequences

	Sequence		Length	Mass (Da)
	A5VNA8 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 70CAF4B8F47A8E9C	FASTA	238	25,907
	10 20 30 40 50 60 MRPSKRAADE MRAISFERGV SKHAEGSCLV KFGDTHVLCT ASLEEKVPGW MRNTGKGWVT 70 80 90 100 110 120 AEYGMLPRST GERMRREAAA GKQGGRTQEI QRLIGRSLRA VVDMQALGEM QITVDCDVIQ 130 140 150 160 170 180 ADGGTRTAAI TGGWVALHEC LRWMEARQMV RVEKVLKDHV AAISCGIYEG VPVLDLDYAE 190 200 210 220 230 DSVAETDSNF VMTGKGGIVE IQGTAEGVPF SEEEFGALMK LARSGIDRLV SLQKMAVA

References

[1]	Paulsen I. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25840 / 63/290 / NCTC 10512.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000708 Genomic DNA. Translation: ABQ60510.1.
RefSeq	YP_001258205.1. NC_009505.1.
3D structure databases
ProteinModelPortal	A5VNA8.
SMR	A5VNA8. Positions 2-238.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VNA8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5202778.
GenomeReviews	Gene locus BOV_0167 in contig CP000708_GR.
KEGG	bov:BOV_0167.
PATRIC	17860058. VBIBruOvi136990_1453.
TIGR	BOV_0167.
Phylogenomic databases
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_BRUO2

Accession

Primary (citable) accession number: A5VNA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program