Reviewed, UniProtKB/Swiss-Prot A5VMP8 (ASNA_LACRD)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	Lreu_1885

Organism

Lactobacillus reuteri (strain DSM 20016) [Complete proteome] [HAMAP]

Taxonomic identifier

557436 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000061106

Sequences

	Sequence		Length	Mass (Da)
	A5VMP8-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: A861C4CA19CF5042	FASTA	336	39,389
	10 20 30 40 50 60 MDLTIPKDYD PRLSIRETEA AIRYIRETFQ DEFGKELNLQ RMSAPMFVEK STGLNDNLNG 70 80 90 100 110 120 VEQPVSFEMK DMPNETVEIV HSLAKWKRLA LKRYGFGMHE GLYTNMNAIR KEEDLDNFHS 130 140 150 160 170 180 IYVDQWDWEK IISKDERTEE TLKDTVRDIF KVIKHMEHEV WYKFPQAVYH LPDEIHFVTT 190 200 210 220 230 240 QELEDRWPDL TPLEREDKIA KELGAVFVMK IGDKLQRSGK PHDGRAPDYD DWSLNGDIIF 250 260 270 280 290 300 WYEPLQRRIE VSSMGIRVSP ESMKYQLEQA DATDREKLPF HKMLLNGELP YTIGGGIGQS 310 320 330 RLCMLLLGKA HIGEVQASIW PEDMIKKCEE NHIQIL

References

[1]

"Complete sequence of chromosome of Lactobacillus reuteri DSM 20016." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Walter J., Heng N.C.K., Tannock G.W., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000705 Genomic DNA. Translation: ABQ84122.1.
RefSeq	YP_001272459.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5190139.
GenomeReviews	Gene locus Lreu_1885 in contig CP000705_GR.
KEGG	lre:Lreu_1885.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LNDNLNG.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_LACRD

Accession

Primary (citable) accession number: A5VMP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 10, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)