A5VLV3 (LUXS_LACRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase
EC=4.4.1.21

Alternative name(s):
AI-2 synthesis protein
Autoinducer-2 production protein LuxS

Gene names

Name:	luxS
Ordered Locus Names:	Lreu_1586

Organism

Lactobacillus reuteri (strain DSM 20016) [Complete proteome] [HAMAP]

Taxonomic identifier

557436 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091
Subunit structure	Homodimer By similarity. HAMAP MF_00091
Sequence similarities	Belongs to the LuxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 158	158	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_1000057608
Sites
	Metal binding	54	1	Iron By similarity
	Metal binding	58	1	Iron By similarity
	Metal binding	124	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5VLV3 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 183C7DDE02A81BD5	FASTA	158	17,695
	10 20 30 40 50 60 MAKVESFTLD HTKVKAPYVR LITVEEGPKG DKISNYDLRL VQPNENAIPT AGLHTIEHLL 70 80 90 100 110 120 AGLLRDRLGG VIDCSPFGCR TGFHLITWGE HSTTEVAKAL KSSLEEIAYK TKWEDVQGTT 130 140 150 IESCGNYRDH SLFSAKEWSK KILDEGISDK PFERHVVD

References

[1]

"The evolution of host specialization in the vertebrate gut symbiont Lactobacillus reuteri." Frese S.A., Benson A.K., Tannock G.W., Loach D.M., Kim J., Zhang M., Oh P.L., Heng N.C., Patil P.B., Juge N., Mackenzie D.A., Pearson B.M., Lapidus A., Dalin E., Tice H., Goltsman E., Land M., Hauser L. , Ivanova N., Kyrpides N.C., Walter J. PLoS Genet. 7:E1001314-E1001314(2011) [PubMed: 21379339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 20016.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000705 Genomic DNA. Translation: ABQ83827.1.
RefSeq	YP_001272164.1. NC_009513.1.
3D structure databases
ProteinModelPortal	A5VLV3.
SMR	A5VLV3. Positions 3-150.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VLV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5188910.
GenomeReviews	Gene locus Lreu_1586 in contig CP000705_GR.
KEGG	lre:Lreu_1586.
PATRIC	22256432. VBILacReu87937_1604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG347473.
OMA	TTFDIRM.
ProtClustDB	PRK02260.
Enzyme and pathway databases
BioCyc	LREU557436:LREU_1586-MONOMER.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
KO	K07173.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LUXS_LACRD

Accession

Primary (citable) accession number: A5VLV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program