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A5VL28 (SYL_LACRD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Lreu_1295
OrganismLactobacillus reuteri (strain DSM 20016) [Complete proteome] [HAMAP]
Taxonomic identifier557436 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length806 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 806806Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000057345

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif578 – 5825"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5811ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5VL28 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 08383790E3207E6C

FASTA80692,919
        10         20         30         40         50         60 
MAYDHKTIEK KWQKFWKKNE TFKADLNKDQ KKYYALDMFP YPSGQGLHVG HPEGYTATDV 

        70         80         90        100        110        120 
MSRMKRMQGF NVLHPMGWDA FGLPAEQYAL KTGHNPKDFT NKNIDHFRDQ IQSLGFSYDW 

       130        140        150        160        170        180 
DREVNTTDPK FYKWTQWIFE QLYKKGLAYE SEIMVNWAPD FMGGTVVANE EVEDGKTKRG 

       190        200        210        220        230        240 
GYPVYRKPMR QWVLKITAYA DRLIDDLDLV DWPESVKEMQ RNWIGRSEGA SVFFPVVGDE 

       250        260        270        280        290        300 
DTKIEVFTTR ADTLFGASYV VLAPEQELVD QLTTPEHKAE VEKYKEEASR RSDLERTDLN 

       310        320        330        340        350        360 
KDKTGVFTGS YVINPVNGEK LPIWISDYVL ASYGTGAVMA VPSGDQRDYD FATKFNLPIK 

       370        380        390        400        410        420 
PIIEGADISE GAFDGDGKHI NSGFLDGLNI ADAKQKMIDW LEEHDAGHKK VNYRLRDWIF 

       430        440        450        460        470        480 
SRQRYWGEPI PVIHWDDGTT SLVPEDELPL ELPKTDNIEP SGTGESPLAN VEDWVNVYDE 

       490        500        510        520        530        540 
NGRHGLRETN TMPQWAGSSW YWLRYTDPHN DEEFASKEAL DYWSPVDLYV GGAEHAVLHL 

       550        560        570        580        590        600 
LYARFWHKVL YDLGLVPTKE PFMKLVNQGM ILGSNHEKMS KSKGNVVNPD DIVDQYGADT 

       610        620        630        640        650        660 
LRLYEMFMGP LEESVPWDEK GLHGANKWVQ RVWRLLMDDN NHLRDRVSTF NDGKLTKVYN 

       670        680        690        700        710        720 
QTVKKVTEDY ERMHFNTAIS QLMVFVNEAY KVDDLPVEYM KGFVKMIAPI MPHMAEELWS 

       730        740        750        760        770        780 
QFGESDTITY QPWPTYDPKA LVEDEVEMIV QVNGKVRAKI KMAKDTDRDE AQQLALANEH 

       790        800 
VKKFTDGKDI KKVIVVPNKI VNIVAK 

« Hide

References

[1]"The evolution of host specialization in the vertebrate gut symbiont Lactobacillus reuteri."
Frese S.A., Benson A.K., Tannock G.W., Loach D.M., Kim J., Zhang M., Oh P.L., Heng N.C., Patil P.B., Juge N., Mackenzie D.A., Pearson B.M., Lapidus A., Dalin E., Tice H., Goltsman E., Land M., Hauser L. expand/collapse author list , Ivanova N., Kyrpides N.C., Walter J.
PLoS Genet. 7:E1001314-E1001314(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 20016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000705 Genomic DNA. Translation: ABQ83552.1.
RefSeqYP_001271889.1. NC_009513.1.

3D structure databases

ProteinModelPortalA5VL28.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557436.Lreu_1295.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ83552; ABQ83552; Lreu_1295.
GeneID5188374.
KEGGlre:Lreu_1295.
PATRIC22255798. VBILacReu87937_1309.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLREU557436:GC7Y-1358-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACRD
AccessionPrimary (citable) accession number: A5VL28
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries