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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Lactobacillus reuteri (strain DSM 20016)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441S-adenosyl-L-methionineUniRule annotation
Binding sitei69 – 691S-adenosyl-L-methionineUniRule annotation
Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
Active sitei118 – 1181By similarity
Binding sitei118 – 1181S-adenosyl-L-methionineUniRule annotation
Binding sitei122 – 1221SubstrateUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLREU557436:GC7Y-1321-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Lreu_1260
OrganismiLactobacillus reuteri (strain DSM 20016)
Taxonomic identifieri557436 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001991 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213tRNA (guanine-N(7)-)-methyltransferasePRO_1000064399Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA5VKZ3.
SMRiA5VKZ3. Positions 9-212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 1954Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5VKZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVKHKKWAD PLIAAHPELM IDDATQFKGK WQSRFAKEQP LHLEVGMGKG
60 70 80 90 100
QFIIGMAKDH PEINFIGLEI QRTVAAIALK KALEEDLPNL QLICGDGEDL
110 120 130 140 150
QEYFEDGEVA KMYLNFSDPW PKKRHAKRRL TYKTFLATYQ QILQDQGAIE
160 170 180 190 200
LKTDNMGLFE FSLESMNNYG MIFDGVWLDL HHSEENEHNV ETEYEQKFAA
210
KGQPIYKLIA NFK
Length:213
Mass (Da):24,675
Last modified:July 10, 2007 - v1
Checksum:i4858D127CE955457
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000705 Genomic DNA. Translation: ABQ83517.1.
RefSeqiWP_003668507.1. NC_009513.1.
YP_001271854.1. NC_009513.1.

Genome annotation databases

EnsemblBacteriaiABQ83517; ABQ83517; Lreu_1260.
GeneIDi5188634.
KEGGilre:Lreu_1260.
PATRICi22255724. VBILacReu87937_1274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000705 Genomic DNA. Translation: ABQ83517.1.
RefSeqiWP_003668507.1. NC_009513.1.
YP_001271854.1. NC_009513.1.

3D structure databases

ProteinModelPortaliA5VKZ3.
SMRiA5VKZ3. Positions 9-212.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ83517; ABQ83517; Lreu_1260.
GeneIDi5188634.
KEGGilre:Lreu_1260.
PATRICi22255724. VBILacReu87937_1274.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciLREU557436:GC7Y-1321-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 20016.

Entry informationi

Entry nameiTRMB_LACRD
AccessioniPrimary (citable) accession number: A5VKZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: June 24, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.