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A5VK99 (THIM_LACRD) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:Lreu_1013
OrganismLactobacillus reuteri (strain DSM 20016) [Complete proteome] [HAMAP]
Taxonomic identifier557436 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383872

Sites

Binding site461Substrate; via amide nitrogen By similarity
Binding site1211ATP By similarity
Binding site1661ATP By similarity
Binding site1931Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A5VK99 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 977DD70D51796B89

FASTA26929,028
        10         20         30         40         50         60 
MVQRQINWSL IDRVRAKNPI VLNLANLVTI DKVADAVSAV GASPIMSVEP AEADEMVMLA 

        70         80         90        100        110        120 
NALSINLGTI NEHQATQIRT VLRAATPLKP LVLDPVAVSA VPSRLKFAHS LLNDFHFDVI 

       130        140        150        160        170        180 
RGNASEIAAL VEADNTSHGI DAGKVPNQVQ IAETCARRYH SIVVLTGETD IITDGQVVYE 

       190        200        210        220        230        240 
NPFSAEMLTM NVGSGDMLSS IIAAFLGITT NTWDACIVAT VLVSAAGVLA NRYSVGLGSW 

       250        260 
QVQFFDQLSI MDTKALLEFF DESEEDYLD 

« Hide

References

[1]"The evolution of host specialization in the vertebrate gut symbiont Lactobacillus reuteri."
Frese S.A., Benson A.K., Tannock G.W., Loach D.M., Kim J., Zhang M., Oh P.L., Heng N.C., Patil P.B., Juge N., Mackenzie D.A., Pearson B.M., Lapidus A., Dalin E., Tice H., Goltsman E., Land M., Hauser L. expand/collapse author list , Ivanova N., Kyrpides N.C., Walter J.
PLoS Genet. 7:E1001314-E1001314(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 20016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000705 Genomic DNA. Translation: ABQ83273.1.
RefSeqYP_001271610.1. NC_009513.1.

3D structure databases

ProteinModelPortalA5VK99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING557436.Lreu_1013.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ83273; ABQ83273; Lreu_1013.
GeneID5188508.
KEGGlre:Lreu_1013.
PATRIC22255210. VBILacReu87937_1021.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAQTFTANV.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycLREU557436:GC7Y-1070-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_LACRD
AccessionPrimary (citable) accession number: A5VK99
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways