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Protein

Argininosuccinate synthase

Gene

argG

Organism
Lactobacillus reuteri (strain DSM 20016)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861CitrullineUniRule annotation
Binding sitei116 – 1161ATP; via amide nitrogenUniRule annotation
Binding sitei118 – 1181AspartateUniRule annotation
Binding sitei122 – 1221AspartateUniRule annotation
Binding sitei122 – 1221CitrullineUniRule annotation
Binding sitei123 – 1231AspartateUniRule annotation
Binding sitei126 – 1261CitrullineUniRule annotation
Binding sitei174 – 1741CitrullineUniRule annotation
Binding sitei259 – 2591CitrullineUniRule annotation
Binding sitei271 – 2711CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 179ATPUniRule annotation

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLREU557436:GC7Y-778-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Lreu_0731
OrganismiLactobacillus reuteri (strain DSM 20016)
Taxonomic identifieri557436 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001991: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Argininosuccinate synthasePRO_1000057039Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi557436.Lreu_0731.

Structurei

3D structure databases

ProteinModelPortaliA5VJH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5VJH1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKEKIVLAY SGGLDTSVAI AWLKNKGYDV IACCIDVGEG KDLEAIKEKG
60 70 80 90 100
LQVGAWKSVV IDAKRDFAEQ FVLPALQAHA MYEQKYPLVS ALSRPLIVQK
110 120 130 140 150
LVAVANQYGA TAIAHGCTGK GNDQVRFEAG IHALAPEMKI EDPIRDWHWS
160 170 180 190 200
REEEIQYAKD NGIPVPITKA SPYSIDENLW GRANECGILE DPWAAAPADA
210 220 230 240 250
YDRTVSIEEA PDTPTTIEIT FNEGVPTAID GEEMPLDQLI MKLDKLAGSH
260 270 280 290 300
GIGRIDHVEN RLVGIKSREI YECPAATVLL AAHKDLEDLT QEREVAHFKP
310 320 330 340 350
LIEQKMSGII YNGLWYSPLM KSLVAFIDES QAVVNGVVRV KLFKGNVICE
360 370 380 390 400
GRKSPNSLYD KNLATYTSAD EFDQEAATGF IKLWELPDKV YAQVQNKNKK
410
KVKENTSDAY
Length:410
Mass (Da):45,395
Last modified:July 10, 2007 - v1
Checksum:iEF62608A1AFC3D92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000705 Genomic DNA. Translation: ABQ82995.1.
RefSeqiYP_001271332.1. NC_009513.1.

Genome annotation databases

EnsemblBacteriaiABQ82995; ABQ82995; Lreu_0731.
GeneIDi5189344.
KEGGilre:Lreu_0731.
PATRICi22254637. VBILacReu87937_0745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000705 Genomic DNA. Translation: ABQ82995.1.
RefSeqiYP_001271332.1. NC_009513.1.

3D structure databases

ProteinModelPortaliA5VJH1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi557436.Lreu_0731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ82995; ABQ82995; Lreu_0731.
GeneIDi5189344.
KEGGilre:Lreu_0731.
PATRICi22254637. VBILacReu87937_0745.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciLREU557436:GC7Y-778-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 20016.

Entry informationi

Entry nameiASSY_LACRD
AccessioniPrimary (citable) accession number: A5VJH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: February 4, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.