A5VI11 (ISPE_LACRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Lreu_0215

Organism

Lactobacillus reuteri (strain DSM 20016) [Complete proteome] [HAMAP]

Taxonomic identifier

557436 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000057422
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	137	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5VI11 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: E48531AC25B43759	FASTA	283	31,666
	10 20 30 40 50 60 MIVTEKAPAK LNLSLDTPMR YFDGSPQWDM VMVSADLADY VTVETHRRPA TIKVYTNSGF 70 80 90 100 110 120 LPNDQRNLAY QAAHILRSRF HCKDGVTIRI KKQIPVAAGL GGGSSDAAAV LRALNSIWRL 130 140 150 160 170 180 GLSLSELAKI ALTIDSDVPY CIYNKLAHVT GHGEKIELLP PQPHYWAVIA KQKISVSTPQ 190 200 210 220 230 240 ILRQINYEKL QHLNNEALLT NLKKEDWQEA TKYMGNVLEP LTMKFYPEIG RLKNKMKELG 250 260 270 280 ADVAQMSGTG PTVFAICHTE SRAKRIQNSI RGFCRDVHVV TLL

References

[1]

"The evolution of host specialization in the vertebrate gut symbiont Lactobacillus reuteri." Frese S.A., Benson A.K., Tannock G.W., Loach D.M., Kim J., Zhang M., Oh P.L., Heng N.C., Patil P.B., Juge N., Mackenzie D.A., Pearson B.M., Lapidus A., Dalin E., Tice H., Goltsman E., Land M., Hauser L. , Ivanova N., Kyrpides N.C., Walter J. PLoS Genet. 7:E1001314-E1001314(2011) [PubMed: 21379339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 20016.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000705 Genomic DNA. Translation: ABQ82485.1.
RefSeq	YP_001270822.1. NC_009513.1.
3D structure databases
ProteinModelPortal	A5VI11.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VI11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5189027.
GenomeReviews	Gene locus Lreu_0215 in contig CP000705_GR.
KEGG	lre:Lreu_0215.
PATRIC	22253512. VBILacReu87937_0224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
ProtClustDB	PRK00128.
Enzyme and pathway databases
BioCyc	LREU557436:LREU_0215-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_LACRD

Accession

Primary (citable) accession number: A5VI11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program