A5VFQ7 (A5VFQ7_SPHWW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172

Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172

Gene names

Name:	metE HAMAP MF_00172
Ordered Locus Names:	Swit_4786

Organism

Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) [Complete proteome] [HAMAP]

Taxonomic identifier

392499 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis HAMAP MF_00172 SAAS SAAS013215
Domain	Repeat HAMAP MF_00172
Ligand	Metal-binding HAMAP MF_00172 SAAS SAAS013215 Zinc HAMAP MF_00172 SAAS SAAS013215
Molecular function	Methyltransferase HAMAP MF_00172 SAAS SAAS013215 EMBL ABQ71123.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	659	1	Zinc By similarity HAMAP MF_00172
	Metal binding	661	1	Zinc By similarity HAMAP MF_00172
	Metal binding	744	1	Zinc By similarity HAMAP MF_00172

Sequences

	Sequence		Length	Mass (Da)
	A5VFQ7 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: FF65405E8A812B7E	FASTA	773	85,285
	10 20 30 40 50 60 MSVTAANLGF PRIGPRRELK TAVEAYWAGK ADEAQLLDTA RRLRAATWDR QKAAGIAVIP 70 80 90 100 110 120 SNDFSLYDQV LDTSVMVGAI PDIYRGLGAP DSLAVYFAMA RGTRTADDDA GCAHGHGKGG 130 140 150 160 170 180 DVPAQEMTKW FDTNYHYMVP EVSPDQGFAL ATAKPVDHFR EARALGHHTR PVILGPVTWL 190 200 210 220 230 240 LLAKSKAEGF DPLSLLPRLL PVYADLLGRL RDAGADWVQI DEPALVLDLS DAARAAYVSA 250 260 270 280 290 300 YGELAKVDGI KLMLATYFGA LGDNLKTALS LPVAGLHVDL VRAPEQLDAV LAGARKDLLL 310 320 330 340 350 360 SLGLIDGRNI WKADLTALLD RYEPIMKSRS AFQIAPSCSL LHTPIDLALE TKLDPQVKDW 370 380 390 400 410 420 LAFAAQKLEE LSLLGLGLSN GRYVISDELL ANADSIQGRR NSPLTNDRKV RDRAARITGD 430 440 450 460 470 480 WARRKAPFAD RIKAQQARLN LPLLPTTTIG SFPQTAEVRK ARSAHDKGEL DTAGYERFLR 490 500 510 520 530 540 EETARAIAWQ EEIGIDVLVH GEFERNDMVQ YFGEQLAGFA FSRAGWVQSY GSRCVRPPII 550 560 570 580 590 600 YGDVSRPKPM TVAWWHYAQS VTARPMKGML TGPVTILNWS FVRDDQSREE TCRQIALAIR 610 620 630 640 650 660 DEVLDLEAAG ARIIQIDEAA LREGLPLRRA DWQHYLDWAV ESFRISAAGV ADETQIHTHM 670 680 690 700 710 720 CYSEFNEIID SIGAMDADVI SIETSRSKME LLDAFVDYAY PNEIGPGVYD IHSPRIPTTR 730 740 750 760 770 EMEALLIKAG ERLSARQLWV NPDCGLKTRK WEDVKPALVA MVDAAKAARA ALA

References

[1]

"Complete sequence of chromosome of Sphingomonas wittichii RW1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Halden R.U., Miller T.R., Salzberg S.L., Eisen J.A., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RW1 / DSM 6014 / JCM 10273.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000699 Genomic DNA. Translation: ABQ71123.1.
RefSeq	YP_001265261.1. NC_009511.1.
3D structure databases
ProteinModelPortal	A5VFQ7.
SMR	A5VFQ7. Positions 5-770.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VFQ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5199329.
GenomeReviews	Gene locus Swit_4786 in contig CP000699_GR.
KEGG	swi:Swit_4786.
NMPDR	fig|392499.4.peg.4793.
PATRIC	23688891. VBISphWit55028_5367.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5VFQ7_SPHWW

Accession

Primary (citable) accession number: A5VFQ7

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)