A5VDT3 (ISPE_SPHWW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Swit_4106

Organism

Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) [Complete proteome] [HAMAP]

Taxonomic identifier

392499 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335759
Regions
	Nucleotide binding	109 – 119	11	ATP Potential
Sites
	Active site	23	1	By similarity
	Active site	151	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5VDT3 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 883C78DBC08F612B	FASTA	293	30,578
	10 20 30 40 50 60 MTVGSGHRWP EPSMHPTEIA YAKINLALHV RRRRADGYHD IETIFAFAEH GDIVAAEPGP 70 80 90 100 110 120 ARLMLAGPFA PALAGEDPED NLVMRAADGL CALAGEAARP AIRLDKRLPV AAGIGGGSAD 130 140 150 160 170 180 AAAVLRLLGR AYGIAADDPR VMTLAATLGA DVPACVRSAT VRGGERGDRL ESIAADGLHG 190 200 210 220 230 240 LPLLLVNPRV ALPTGPVFAA WDRVDRGPLA PGDPLAAALA GRNDLEAPAT LLHPVIGEVV 250 260 270 280 290 TLLAAQPGAI LARMSGSGAT CFALFGSEAD RDAADRALAH RRPDWWRLTS RLR

References

[1]

"Complete sequence of chromosome of Sphingomonas wittichii RW1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Halden R.U., Miller T.R., Salzberg S.L., Eisen J.A., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RW1 / DSM 6014 / JCM 10273.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000699 Genomic DNA. Translation: ABQ70449.1.
RefSeq	YP_001264587.1. NC_009511.1.
3D structure databases
ProteinModelPortal	A5VDT3.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VDT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5199305.
GenomeReviews	Gene locus Swit_4106 in contig CP000699_GR.
KEGG	swi:Swit_4106.
NMPDR	fig|392499.4.peg.4179.
PATRIC	23687505. VBISphWit55028_4681.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	KINLHLG.
ProtClustDB	PRK14608.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_SPHWW

Accession

Primary (citable) accession number: A5VDT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program