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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851MagnesiumUniRule annotation
Metal bindingi491 – 4911MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciSWIT392499:GHZK-3856-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Swit_3810
OrganismiSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Taxonomic identifieri392499 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas
ProteomesiUP000001989 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 774774Polyribonucleotide nucleotidyltransferasePRO_0000329859Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi392499.Swit_3810.

Structurei

3D structure databases

ProteinModelPortaliA5VCY9.
SMRiA5VCY9. Positions 616-689.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61160KHUniRule annotationAdd
BLAST
Domaini621 – 68969S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5VCY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDTKKVEIQ WGGQTLTLET GRVARQADGA VLATLGETVV LCAVTAARSV
60 70 80 90 100
KEGQDFFPLT VHYQEKYFSS GRIPGGFFKR ERGATEKETL VSRLIDRPIR
110 120 130 140 150
PLFPEGFYNE INVIAQVLSY DGENEPDILA MIAASAALTI SGVPFMGPIG
160 170 180 190 200
AARVGYKDGE YLLNPTDADV AAGDLDLVVA ATHDAVMMVE SEAKELSEEV
210 220 230 240 250
MLGAVMFAHK ASQDVIKAII KLAEKSAKDP WEMAPQADLS AAKTKLKKLV
260 270 280 290 300
GKDITAAYKL TNKSARSSAL NEARAKAKEA FADATPQDQM AAIKLVKKLE
310 320 330 340 350
AEIVRTAILK DGRRIDGRDT RTVRPIVAEA HFLPRAHGSA LFTRGETQSI
360 370 380 390 400
STCTLGTKES EQMIDGLNGL RYEHFMLHYN FPPYSVGEVG RFGAPGRREV
410 420 430 440 450
GHGKLAWRAL HAVLPTKEEF PYTIRLTSDI TESNGSSSMA TVCGGSLAMM
460 470 480 490 500
DAGVPIKRPV SGIAMGLILE GKDFAVISDI LGDEDHLGDM DFKVAGTSEG
510 520 530 540 550
ITTMQMDIKI AGITEEIFKT ALLQAKEGRA HILGEMAKAL DHTRTELSAH
560 570 580 590 600
APRIETMSVP KDKIRDIIGT GGKIIREIVA TTGAKVDIDD DGTVKISSSD
610 620 630 640 650
TAQIEAARNW IIGIVAEPEV GKIYTGKVVN LVDFGAFVNF MGGKDGLVHV
660 670 680 690 700
SEIKNERVEK VADALSEGQE VKVKVLEIDN RGKVRLSMRV VDQETGEELP
710 720 730 740 750
DTRPPREERP RGDRGDRGDR GPRRDGDRRR EGGDRGPRRD RGDRGDRPRR
760 770
ERSEGGDEGP APDFAPAFLT RDDD
Length:774
Mass (Da):84,203
Last modified:July 10, 2007 - v1
Checksum:i3935AAA71652CD47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000699 Genomic DNA. Translation: ABQ70155.1.
RefSeqiWP_012049994.1. NC_009511.1.

Genome annotation databases

EnsemblBacteriaiABQ70155; ABQ70155; Swit_3810.
KEGGiswi:Swit_3810.
PATRICi23686879. VBISphWit55028_4371.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000699 Genomic DNA. Translation: ABQ70155.1.
RefSeqiWP_012049994.1. NC_009511.1.

3D structure databases

ProteinModelPortaliA5VCY9.
SMRiA5VCY9. Positions 616-689.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi392499.Swit_3810.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ70155; ABQ70155; Swit_3810.
KEGGiswi:Swit_3810.
PATRICi23686879. VBISphWit55028_4371.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciSWIT392499:GHZK-3856-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RW1 / DSM 6014 / JCM 10273.

Entry informationi

Entry nameiPNP_SPHWW
AccessioniPrimary (citable) accession number: A5VCY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 10, 2007
Last modified: July 22, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.