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Protein

Thymidylate synthase

Gene

thyA

Organism
Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26dUMPUniRule annotation1
Active sitei190NucleophileUniRule annotation1
Binding sitei2135,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei221dUMPUniRule annotation1
Binding sitei3075,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi170 – 171dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi210 – 213dUMPUniRule annotation4
Nucleotide bindingi251 – 253dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Swit_3787
OrganismiSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Taxonomic identifieri392499 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas
Proteomesi
  • UP000001989 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003214831 – 308Thymidylate synthaseAdd BLAST308

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi392499.Swit_3787

Structurei

3D structure databases

ProteinModelPortaliA5VCW6
SMRiA5VCW6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257899
KOiK00560
OMAiKQYLDLC
OrthoDBiPOG091H02L6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

A5VCW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEHPEQQYL DAMARAWYGG DERVDRTGVG TKSLFGVTMR FDLSDGTVPL
60 70 80 90 100
ITTKKIFWKS AIKELIWFLS GDTNIRPLVM QNVHIWTDWP LDKYRKATGE
110 120 130 140 150
AIDRDAFERR IVDDPAFAAE WGDLGPVYGK QWVDWPRYTP AGDGLFRREE
160 170 180 190 200
KGVNQIAELV GMLRANPSSR RLIFTGWNVP ELPGMALPPC HMTYQYHVAG
210 220 230 240 250
GRLSGILYQR SCDLGLGFPF NIFEAAVLIR MLAQQADLEP GELVWMGADT
260 270 280 290 300
HVYSNHGHLV EEQLSRSPRP FPRLSLARKP ADMFSYALDD FVIEGYDPHP

HIKAEVAV
Length:308
Mass (Da):34,966
Last modified:July 10, 2007 - v1
Checksum:i66AD3DFB498742B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000699 Genomic DNA Translation: ABQ70132.1
RefSeqiWP_012049971.1, NC_009511.1

Genome annotation databases

EnsemblBacteriaiABQ70132; ABQ70132; Swit_3787
KEGGiswi:Swit_3787

Similar proteinsi

Entry informationi

Entry nameiTYSY_SPHWW
AccessioniPrimary (citable) accession number: A5VCW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 10, 2007
Last modified: April 25, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health