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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei248ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:Swit_3213
OrganismiSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Taxonomic identifieri392499 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas
Proteomesi
  • UP000001989 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309991 – 474Glutamate--tRNA ligase 2Add BLAST474

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi392499.Swit_3213

Structurei

3D structure databases

ProteinModelPortaliA5VB94
SMRiA5VB94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi16 – 26"HIGH" regionUniRule annotationAdd BLAST11
Motifi245 – 249"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A5VB94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGASMEKQTV VTRFAPSPTG FLHIGGARTA LFNWLFARHH GGKFLLRIED
60 70 80 90 100
TDRARSTQEA IDAILDGMRW LDLGWDGEVV YQFARAERHA EVARELLANG
110 120 130 140 150
HAYRCYATPE ELAELREQQR AAKQPLRYDG RWRDRDPSEA PAGAPFVIRL
160 170 180 190 200
KAPRTGEVTI DDQVQGPVTV QNAELDDMIL LRSDGTPTYM LAVVVDDHDM
210 220 230 240 250
GVTHVIRGDD HLNNAFRQLA LIRAMGWEEP VYAHIPLIHG ADGAKLSKRH
260 270 280 290 300
GALGVDAYRD ELGLLSEAVN NYLLRLGWGH GDDEIISREQ AVEWFDLAGV
310 320 330 340 350
GRSPSRFDFK KLENLNGHYM READDARLAD LIAPGVAARA GREFDSADHD
360 370 380 390 400
LLLRSIPFLK VRAKDINELA EGASFLFRTR PLDMDEKAAS LLDDPGKALL
410 420 430 440 450
AQAREALAAT DDWSALALEA GVRSVAERGG IGLGKVAQPL RAALTGRTTS
460 470
PGIFDVLALL GREESLGRLD DQLG
Length:474
Mass (Da):52,244
Last modified:July 10, 2007 - v1
Checksum:i485B320F77089633
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000699 Genomic DNA Translation: ABQ69560.1

Genome annotation databases

EnsemblBacteriaiABQ69560; ABQ69560; Swit_3213
KEGGiswi:Swit_3213

Similar proteinsi

Entry informationi

Entry nameiSYE2_SPHWW
AccessioniPrimary (citable) accession number: A5VB94
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 10, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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