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A5VA89 (SYL_SPHWW) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Swit_2852
OrganismSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) [Complete proteome] [HAMAP]
Taxonomic identifier392499 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334823

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif612 – 6165"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6151ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5VA89 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: D328A1D3BF136638

FASTA83592,289
        10         20         30         40         50         60 
MSARFNHLKA DAHWQGVWES QGTFQARDDS PKPKSYVLEM FPYPSGRIHM GHVRNYTMGD 

        70         80         90        100        110        120 
VLARYRRMKG FEVLHPMGWD AFGMPAENAA MEKKVHPGDW TRANIAAMRD QLKRIGFALD 

       130        140        150        160        170        180 
WSRELATCEP DYYGQEQALF LDLYAAGLVY RKESAVNWDP VDMTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVERRKLNQ WFLKITDFAD DLLDGLDTLD QWPEKVRTMQ ENWIGKSQGM RFTFVLDAPA 

       250        260        270        280        290        300 
GDLTSFDVFT TRPDTMFGAS FAAIAADHPI AQALSAGNPA LQAFIADCKR TGTAAAEIET 

       310        320        330        340        350        360 
AEKKGYDTGL SVVHPLDPDW KLPLFVANFV LMDYGTGAVF GCPAHDQRDL DFARKYALPV 

       370        380        390        400        410        420 
KRVVAPSPEE AAAPIGDEAY VGPGRIVNSA FLDGLSVEEA KAAVIARAEA GGWGQGTTVW 

       430        440        450        460        470        480 
RLRDWGVSRQ RYWGTPIPII HCEGCGAVPV PHDQLPVVLP EDVAFDIPGN PLDRHPTWKH 

       490        500        510        520        530        540 
VDCPSCGKPA RRETDTLDTF VDSSWYFIRF ASQPSDKPFD RAVAESWMPV GQYIGGVEHA 

       550        560        570        580        590        600 
ILHLLYARFW TRALERIGRI GVKEPFTGLF TQGMVTHETY KDPAGQWLSP EEVSDGIVVA 

       610        620        630        640        650        660 
TGAPATVGRI EKMSKSKKNV VDPAPIVEQY GADAVRWFML SDSPPERDLE WTESGIEGCW 

       670        680        690        700        710        720 
RFVNRLWRIT DVSEAGDGED RELDRKLHKA IAGIAADIEA LGFNRAVAKI HELSNAIEKA 

       730        740        750        760        770        780 
APSASRAAAA RALVRLVAPM VPHLAEEAWA RLGEQGLVAD AAWPAHDPAL LVDDEVTIAV 

       790        800        810        820        830 
QVNGKLRDTL TAPKGAPKDA LERMALESEK ITRLLEGAAP RKVIVVPDRL VNIVA 

« Hide

References

[1]"Complete sequence of chromosome of Sphingomonas wittichii RW1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Halden R.U., Miller T.R., Salzberg S.L., Eisen J.A., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RW1 / DSM 6014 / JCM 10273.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000699 Genomic DNA. Translation: ABQ69205.1.
RefSeqYP_001263343.1. NC_009511.1.

3D structure databases

ProteinModelPortalA5VA89.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING392499.Swit_2852.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ69205; ABQ69205; Swit_2852.
GeneID5197900.
KEGGswi:Swit_2852.
PATRIC23684879. VBISphWit55028_3383.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSWIT392499:GHZK-2886-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SPHWW
AccessionPrimary (citable) accession number: A5VA89
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries