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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei615 – 6151ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSWIT392499:GHZK-2886-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Swit_2852
OrganismiSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Taxonomic identifieri392499 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas
ProteomesiUP000001989 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Leucine--tRNA ligasePRO_0000334823Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi392499.Swit_2852.

Structurei

3D structure databases

ProteinModelPortaliA5VA89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi612 – 6165"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5VA89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARFNHLKA DAHWQGVWES QGTFQARDDS PKPKSYVLEM FPYPSGRIHM
60 70 80 90 100
GHVRNYTMGD VLARYRRMKG FEVLHPMGWD AFGMPAENAA MEKKVHPGDW
110 120 130 140 150
TRANIAAMRD QLKRIGFALD WSRELATCEP DYYGQEQALF LDLYAAGLVY
160 170 180 190 200
RKESAVNWDP VDMTVLANEQ VIDGRGWRSG ALVERRKLNQ WFLKITDFAD
210 220 230 240 250
DLLDGLDTLD QWPEKVRTMQ ENWIGKSQGM RFTFVLDAPA GDLTSFDVFT
260 270 280 290 300
TRPDTMFGAS FAAIAADHPI AQALSAGNPA LQAFIADCKR TGTAAAEIET
310 320 330 340 350
AEKKGYDTGL SVVHPLDPDW KLPLFVANFV LMDYGTGAVF GCPAHDQRDL
360 370 380 390 400
DFARKYALPV KRVVAPSPEE AAAPIGDEAY VGPGRIVNSA FLDGLSVEEA
410 420 430 440 450
KAAVIARAEA GGWGQGTTVW RLRDWGVSRQ RYWGTPIPII HCEGCGAVPV
460 470 480 490 500
PHDQLPVVLP EDVAFDIPGN PLDRHPTWKH VDCPSCGKPA RRETDTLDTF
510 520 530 540 550
VDSSWYFIRF ASQPSDKPFD RAVAESWMPV GQYIGGVEHA ILHLLYARFW
560 570 580 590 600
TRALERIGRI GVKEPFTGLF TQGMVTHETY KDPAGQWLSP EEVSDGIVVA
610 620 630 640 650
TGAPATVGRI EKMSKSKKNV VDPAPIVEQY GADAVRWFML SDSPPERDLE
660 670 680 690 700
WTESGIEGCW RFVNRLWRIT DVSEAGDGED RELDRKLHKA IAGIAADIEA
710 720 730 740 750
LGFNRAVAKI HELSNAIEKA APSASRAAAA RALVRLVAPM VPHLAEEAWA
760 770 780 790 800
RLGEQGLVAD AAWPAHDPAL LVDDEVTIAV QVNGKLRDTL TAPKGAPKDA
810 820 830
LERMALESEK ITRLLEGAAP RKVIVVPDRL VNIVA
Length:835
Mass (Da):92,289
Last modified:July 10, 2007 - v1
Checksum:iD328A1D3BF136638
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000699 Genomic DNA. Translation: ABQ69205.1.
RefSeqiWP_012049055.1. NC_009511.1.
YP_001263343.1. NC_009511.1.

Genome annotation databases

EnsemblBacteriaiABQ69205; ABQ69205; Swit_2852.
KEGGiswi:Swit_2852.
PATRICi23684879. VBISphWit55028_3383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000699 Genomic DNA. Translation: ABQ69205.1.
RefSeqiWP_012049055.1. NC_009511.1.
YP_001263343.1. NC_009511.1.

3D structure databases

ProteinModelPortaliA5VA89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi392499.Swit_2852.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ69205; ABQ69205; Swit_2852.
KEGGiswi:Swit_2852.
PATRICi23684879. VBISphWit55028_3383.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSWIT392499:GHZK-2886-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RW1 / DSM 6014 / JCM 10273.

Entry informationi

Entry nameiSYL_SPHWW
AccessioniPrimary (citable) accession number: A5VA89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 10, 2007
Last modified: April 29, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.