A5VA36 (A5VA36_SPHWW) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase 3 HAMAP MF_01374
EC=3.1.2.6 HAMAP MF_01374

Alternative name(s):
Glyoxalase II 3 HAMAP MF_01374

Gene names

Name:	gloB3 HAMAP MF_01374
Ordered Locus Names:	Swit_2798

Organism

Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) [Complete proteome] [HAMAP]

Taxonomic identifier

392499 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374 SAAS SAAS017782
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374 SAAS SAAS017782
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374 SAAS SAAS017782
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP MF_01374

Ontologies

Keywords
Ligand	Metal-binding HAMAP MF_01374 SAAS SAAS017782 Zinc HAMAP MF_01374 SAAS SAAS017782
Molecular function	Hydrolase HAMAP MF_01374 SAAS SAAS017782 EMBL ABQ69152.1
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	85	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	87	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	89	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	90	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	143	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	162	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	162	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	200	1	Zinc 2 By similarity HAMAP MF_01374

Sequences

	Sequence		Length	Mass (Da)
	A5VA36 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: F8FCBF486B017057	FASTA	272	29,206
	10 20 30 40 50 60 MSRSSCSRLA IGSRPPSPGH RCRIRAIGEM SVEIVRIPVL DDNYVWLLHE PVSGETVVVD 70 80 90 100 110 120 PAVAGPVLAE AERRGWTIGQ IWNTHWHGDH IGGNDAIKAA TGARVSAPAA EAAKIPSLDV 130 140 150 160 170 180 PLREGDSVRI GAVEGMVMEV PAHTAGHIAI HLPDEQAVFV GDTLFAMGCG RLFEGTPAQM 190 200 210 220 230 240 HANMDRLAAL PDATRVYCAH EYTQSNGRYA LAAEPGNGAI RARMERVDAA RARGEATVPT 250 260 270 TIGLERETNP FMRAGSVEEL ARRRAAKDVF RG

References

[1]

"Complete sequence of chromosome of Sphingomonas wittichii RW1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Halden R.U., Miller T.R., Salzberg S.L., Eisen J.A., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RW1 / DSM 6014 / JCM 10273.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000699 Genomic DNA. Translation: ABQ69152.1.
RefSeq	YP_001263290.1. NC_009511.1.
3D structure databases
ProteinModelPortal	A5VA36.
ModBase	Search...
Protein-protein interaction databases
STRING	A5VA36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5196619.
GenomeReviews	Gene locus Swit_2798 in contig CP000699_GR.
KEGG	swi:Swit_2798.
NMPDR	fig|392499.4.peg.2960.
PATRIC	23684767. VBISphWit55028_3327.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	ITHHHRD.
ProtClustDB	CLSK835253.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5VA36_SPHWW

Accession

Primary (citable) accession number: A5VA36

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)