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A5V9Q2 (TRMD_SPHWW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:Swit_2662
OrganismSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) [Complete proteome] [HAMAP]
Taxonomic identifier392499 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas ›

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605
PRO_1000006524

Regions

Region134 – 1396S-adenosyl-L-methionine binding By similarity

Sites

Binding site1141S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A5V9Q2 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 93A1BB4CFD4590F3

FASTA24626,852
        10         20         30         40         50         60 
MTFAATILTL YPEMFPGPLG VSLAGRALAE GKWSCDPVQI RDFATDRHRS VDDTPAGGGA 

        70         80         90        100        110        120 
GMVMRADVLA RAVDHALETR PDSPLLAMTP RGAPLTQARV RQLAAGPGVT ILCGRFEGID 

       130        140        150        160        170        180 
ERLFEARPIE PVSIGDYILS GGEMGALVLL DACIRLLPGV MGAPDSGDEE SFESGLLEYP 

       190        200        210        220        230        240 
HYTRPAEWEG RTIPEVLRSG DHARIEAWRN AMAETDTRLR RPDLWERHEG ARAQSPSGAR 


RQKKER

« Hide

References

[1]"Complete sequence of chromosome of Sphingomonas wittichii RW1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Halden R.U., Miller T.R., Salzberg S.L., Eisen J.A., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RW1 / DSM 6014 / JCM 10273.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000699 Genomic DNA. Translation: ABQ69018.1.
RefSeqYP_001263156.1. NC_009511.1.

3D structure databases

ProteinModelPortalA5V9Q2.
ModBaseSearch...

Protein-protein interaction databases

STRING392499.Swit_2662.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ69018; ABQ69018; Swit_2662.
GeneID5198426.
KEGGswi:Swit_2662.
PATRIC23684487. VBISphWit55028_3187.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0336.
HOGENOMHOG000016242.
KOK00554.
OMAPRLLMSP.
ProtClustDBPRK00026.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_SPHWW
AccessionPrimary (citable) accession number: A5V9Q2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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