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A5V856 (HOA2_SPHWW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-hydroxy-2-oxovalerate aldolase 2
Short name=HOA 2
EC=4.1.3.39
Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase 2
4-hydroxy-2-oxopentanoate aldolase 2
Gene names
Ordered Locus Names:Swit_2113
OrganismSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) [Complete proteome] [HAMAP]
Taxonomic identifier392499 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas ›

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds By similarity. HAMAP-Rule MF_01656

Catalytic activity

4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate. HAMAP-Rule MF_01656

Sequence similarities

Belongs to the 4-hydroxy-2-oxovalerate aldolase family.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandManganese
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processaromatic compound catabolic process

Inferred from electronic annotation. Source: HAMAP

   Molecular_function4-hydroxy-2-oxovalerate aldolase activity

Inferred from electronic annotation. Source: HAMAP

manganese ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3453454-hydroxy-2-oxovalerate aldolase 2 HAMAP-Rule MF_01656
PRO_0000387920

Regions

Region18 – 192Substrate binding By similarity

Sites

Active site221Proton acceptor Potential
Metal binding191Manganese By similarity
Metal binding2011Manganese; via tele nitrogen By similarity
Metal binding2031Manganese; via tele nitrogen By similarity
Binding site1721Substrate By similarity
Binding site2011Substrate By similarity
Binding site2921Substrate By similarity
Site181Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A5V856 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: F10D8B927923165A

FASTA34537,239
        10         20         30         40         50         60 
MSFDVGKDKL YIQDVTLRDG MHAIRHMYGI DHVRAIAQAL DEAGVDAIEV AHGDGLNGAS 

        70         80         90        100        110        120 
FNYGFGAHTD WEWLEAVADV LTRSVLTTLI LPGVGTVDEL RRAYDIGVRS VRVATHCTEA 

       130        140        150        160        170        180 
DVSKQHIGIA RDLGMDVSGF LMMSHMIEPE ALAQQALLME SYGAQCVYVT DSGGALDMDG 

       190        200        210        220        230        240 
VRARFQAYDR VLRPETQRGM HAHHNLSLGV ANSIVAAQQG AVRIDASLAG MGAGAGNAPL 

       250        260        270        280        290        300 
EVFIAAVDRK GWNHGCDVNA LMDAAEDLVR PLQDRPVRVD RETLALGYAG VYSSFLRHAE 

       310        320        330        340 
KAAADYGLDT RTILVELGRR KMVGGQEDMI VDVALDLVRE RGAAA

« Hide

References

[1]"Complete sequence of chromosome of Sphingomonas wittichii RW1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Halden R.U., Miller T.R., Salzberg S.L., Eisen J.A., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RW1 / DSM 6014 / JCM 10273.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000699 Genomic DNA. Translation: ABQ68472.1.
RefSeqYP_001262610.1. NC_009511.1.

3D structure databases

ProteinModelPortalA5V856.
SMRA5V856. Positions 9-338.
ModBaseSearch...

Protein-protein interaction databases

STRING392499.Swit_2113.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ68472; ABQ68472; Swit_2113.
GeneID5197133.
KEGGswi:Swit_2113.
PATRIC23683355. VBISphWit55028_2633.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0119.
HOGENOMHOG000048047.
KOK01666.
OMADIAWIEA.
ProtClustDBPRK08195.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01656. HOA.
InterProIPR017629. 4OH_2_O-val_aldolase.
IPR013785. Aldolase_TIM.
IPR012425. DmpG_comm.
IPR000891. PYR_CT.
[Graphical view]
PANTHERPTHR10277:SF3. PTHR10277:SF3. 1 hit.
PfamPF07836. DmpG_comm. 1 hit.
PF00682. HMGL-like. 1 hit.
[Graphical view]
ProDomPD005364. DmpG_comm. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITEPS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHOA2_SPHWW
AccessionPrimary (citable) accession number: A5V856
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: July 10, 2007
Last modified: May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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