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Protein

Malate dehydrogenase

Gene

mdh

Organism
Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADUniRule annotation
Binding sitei83 – 831SubstrateUniRule annotation
Binding sitei89 – 891SubstrateUniRule annotation
Binding sitei96 – 961NADUniRule annotation
Binding sitei121 – 1211SubstrateUniRule annotation
Binding sitei152 – 1521SubstrateUniRule annotation
Active sitei176 – 1761Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADUniRule annotation
Nucleotide bindingi119 – 1213NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSWIT392499:GHZK-1318-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Swit_1300
OrganismiSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Taxonomic identifieri392499 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas
ProteomesiUP000001989: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Malate dehydrogenasePRO_1000026495Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi392499.Swit_1300.

Structurei

3D structure databases

ProteinModelPortaliA5V5U9.
SMRiA5V5U9. Positions 5-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiDIMQANT.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A5V5U9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTKIALIG AGNIGGTLAH LAASKELGDV VLFDVVEGVP QGKALDLSQC
60 70 80 90 100
GPVEGFDAKL KGTNDYADIA GADVIIVTAG VARKPGMSRD DLLGINLKVM
110 120 130 140 150
KSVGEGIKAN APDAFVICIT NPLDAMVWAL REFSGLPHNK VVGMAGVLDS
160 170 180 190 200
ARFATFLAEE FNVSVQDVTT FVLGGHGDTM VPVVEYSTVA GIPIPDLIKM
210 220 230 240 250
GWSTQERIDA IVQRTRSGGG EIVALLKTGS AFYAPATSAI AMAESYLKDK
260 270 280 290 300
KRVLPCAAYL SGEYGVDDLY VGVPVIIGAN GVEKIVEINL SDSAKANLQV
310 320
SVDAVKELLV ACKGIDSSLA
Length:320
Mass (Da):33,337
Last modified:July 10, 2007 - v1
Checksum:i929A0EDEB34574DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000699 Genomic DNA. Translation: ABQ67665.1.
RefSeqiYP_001261803.1. NC_009511.1.

Genome annotation databases

EnsemblBacteriaiABQ67665; ABQ67665; Swit_1300.
GeneIDi5200794.
KEGGiswi:Swit_1300.
PATRICi23681672. VBISphWit55028_1796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000699 Genomic DNA. Translation: ABQ67665.1.
RefSeqiYP_001261803.1. NC_009511.1.

3D structure databases

ProteinModelPortaliA5V5U9.
SMRiA5V5U9. Positions 5-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi392499.Swit_1300.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ67665; ABQ67665; Swit_1300.
GeneIDi5200794.
KEGGiswi:Swit_1300.
PATRICi23681672. VBISphWit55028_1796.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiDIMQANT.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciSWIT392499:GHZK-1318-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RW1 / DSM 6014 / JCM 10273.

Entry informationi

Entry nameiMDH_SPHWW
AccessioniPrimary (citable) accession number: A5V5U9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: January 7, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.