A5UZF2 (A5UZF2_ROSS1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
EC=6.3.1.5 HAMAP MF_00193

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	RoseRS_3650

Organism

Roseiflexus sp. (strain RS-1) [Complete proteome] [HAMAP] EMBL ABQ92005.1

Taxonomic identifier

357808 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Roseiflexus

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193 SAAS SAAS003694
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193 SAAS SAAS003694
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 SAAS SAAS003694 NAD SAAS SAAS003694 HAMAP MF_00193 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 SAAS SAAS003694 EMBL ABQ92005.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	39 – 46	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	41	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A5UZF2 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: AAA87157D5C958B1	FASTA	345	39,427
	10 20 30 40 50 60 MTFGTHVLQI DAAAETERII AWLQRHVIRT FHRQGVALGI SGGIDSSVAL ALCVRAFGPQ 70 80 90 100 110 120 RVVALMMPER DSDPETLHLS EMVARHYGVE PILEDITPVL EGFGCYRRRD EAVRRIFPEY 130 140 150 160 170 180 NAERGYKMRI VLPQNLLDAD TLNVFSLEIV TPDGEVKSKR LPPREYAQIV AASNFKQRTR 190 200 210 220 230 240 MAMLYYHAEV RNYAVVGTPN KNEHDQGFFV KYGDGGADLR PIVHLYKTQV YQLARYLEVP 250 260 270 280 290 300 EVIQRRPPTS DTYSAPTTQQ EFFFRLPFET MDLLWYAQEH NVSPDDAAQA LGLTPVQVQR 310 320 330 340 AFNDFARKQR TTAYLRMPVL ELRSADQNGE PLKSVEFRRN EGAHT

References

[1]

"Complete sequence of Roseiflexus sp. RS-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000686 Genomic DNA. Translation: ABQ92005.1.
RefSeq	YP_001277955.1. NC_009523.1.
3D structure databases
ProteinModelPortal	A5UZF2.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UZF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5210628.
GenomeReviews	Gene locus RoseRS_3650 in contig CP000686_GR.
KEGG	rrs:RoseRS_3650.
PATRIC	23356015. VBIRosSp109359_4043.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	AHLYKSQ.
ProtClustDB	PRK00876.
Enzyme and pathway databases
BioCyc	RSP357808:ROSERS_3650-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01916.
Pfam	PF02540. NAD_synthase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5UZF2_ROSS1

Accession

Primary (citable) accession number: A5UZF2

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)