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Protein

Chorismate synthase

Gene

aroC

Organism
Roseiflexus sp. (strain RS-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (RoseRS_2785)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481NADPUniRule annotation
Binding sitei54 – 541NADPUniRule annotation
Binding sitei282 – 2821FMN; via amide nitrogenUniRule annotation
Binding sitei323 – 3231FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi125 – 1273FMNUniRule annotation
Nucleotide bindingi297 – 3015FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciRSP357808:GH5Z-1981-MONOMER.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Ordered Locus Names:RoseRS_1952
OrganismiRoseiflexus sp. (strain RS-1)
Taxonomic identifieri357808 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesRoseiflexineaeRoseiflexaceaeRoseiflexus
Proteomesi
  • UP000006554 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Chorismate synthasePRO_1000022542Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi357808.RoseRS_1952.

Structurei

3D structure databases

ProteinModelPortaliA5UUN7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060335.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5UUN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGNTFGQVF RLTTWGESHG PAVGCVVDGC PAGIEISEAF IQRELDRRRV
60 70 80 90 100
GQSRVTSARQ EPDQVQILSG VFEGRSTGAP ISMLVFNTDA KPGHYDTIKH
110 120 130 140 150
LYRPGHADYT WDAKYGFRDW RGGGRSSARE TIGRVAGGAI AKLLLARYGI
160 170 180 190 200
SVIAWTSQLG DLKAEVIDES EIERNIMRCP DARVAALMVE RVEQARRSLD
210 220 230 240 250
SLGGVVEVRA RGVPPGLGEP VFDKLQADIG KAMFSIPAIK GVEFGEGFGV
260 270 280 290 300
AYMTGSTHND PFVRRDDGTI GTASNHHGGI LGGISTGEEI VLRIAAKPPA
310 320 330 340 350
SIARPQHTVD RAGNPAAIEI HGRHDPTVLP RLVPIAEAML ALVLADHLLR

QRLARVDA
Length:358
Mass (Da):38,573
Last modified:July 10, 2007 - v1
Checksum:iA72E28B05EC09B66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000686 Genomic DNA. Translation: ABQ90340.1.
RefSeqiWP_011956686.1. NC_009523.1.

Genome annotation databases

EnsemblBacteriaiABQ90340; ABQ90340; RoseRS_1952.
KEGGirrs:RoseRS_1952.
PATRICi23352196. VBIRosSp109359_2150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000686 Genomic DNA. Translation: ABQ90340.1.
RefSeqiWP_011956686.1. NC_009523.1.

3D structure databases

ProteinModelPortaliA5UUN7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi357808.RoseRS_1952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ90340; ABQ90340; RoseRS_1952.
KEGGirrs:RoseRS_1952.
PATRICi23352196. VBIRosSp109359_2150.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060335.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG6WDSHT.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciRSP357808:GH5Z-1981-MONOMER.

Family and domain databases

Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RS-1.

Entry informationi

Entry nameiAROC_ROSS1
AccessioniPrimary (citable) accession number: A5UUN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: November 11, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.