A5UT75 (A5UT75_ROSS1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ABQ89828.1
EC=5.99.1.2 EMBL ABQ89828.1

Gene names

Ordered Locus Names:

RoseRS_1430

Organism

Roseiflexus sp. (strain RS-1) [Complete proteome] [HAMAP] EMBL ABQ89828.1

Taxonomic identifier

357808 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Roseiflexus

Protein attributes

Sequence length	789 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS005733

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A5UT75 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 06842CF656621B92	FASTA	789	88,342
	10 20 30 40 50 60 MGEKLVIVES PAKARTIQKY LGQGYRVEAS MGHVRDLPKS DLGVDLDANF TPVYEVSPGK 70 80 90 100 110 120 EKIIAALRKS IRQADAVYLA TDPDREGEAI AWHITEAAKI PRGKPVYRVV FTEITPGAVR 130 140 150 160 170 180 SAMASPRDID RRLVDAQQAR RVLDRLVGYK LSPLLWEKVR RGLSAGRVQS VAVRLIVERE 190 200 210 220 230 240 REIEAFVPQE YWTIEADLLK QEARDPFRAV LIERAGRKLE KFDIGSGEQA QAIIVDLEGA 250 260 270 280 290 300 AYIVRKVTRR DKRRSPPPPF TTSTLQQEAG RKLGFSAKRT MAVAQQLYEG VDIGGDEGLV 310 320 330 340 350 360 GLITYMRTDS VNVSREAQEE ARIVIRDRYG EAYLPPKPPV YKTKTKGAQE AHEAIRPTLS 370 380 390 400 410 420 RRTPEQVQPY LTSDQYRLYE LIWKRFVASQ MEAAVFDSTT VDIGAGRGIE RSAGNDPYTF 430 440 450 460 470 480 RTTGSVLKFP GFLAVYNVSL DEGEEDEDSE RRLPLLAEGD VLALVQLLPV QHFTEPPPRF 490 500 510 520 530 540 TEASLVKELE RLGIGRPSTY APTISTIIER EYVELADKKL VPTTLGRVVT DLLVQHFPSI 550 560 570 580 590 600 VDYGFTSEME QQLDDIAEGE KEWTPVLRSF YEPFEQKLAD AQRSMRNVKR EEILTDLTCP 610 620 630 640 650 660 KCSQGQLAIR FGKNGEFLAC NRYPECDFTS DFHRDGEGRI VIDAPAAPEV SDVICELCGR 670 680 690 700 710 720 PMVLKKSRFG PFLGCSGYPE CKNTRRLDKQ GKPVPPPKAT GVTCPKCRQG ELLERRGKFG 730 740 750 760 770 780 RPFFGCSRYP KCDYLVNSLE EVANYTPENN APMEAKAEEQ PATKRTKTST GKKRAKTTAP GSGTPNVED

References

[1]

"Complete sequence of Roseiflexus sp. RS-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000686 Genomic DNA. Translation: ABQ89828.1.
RefSeq	YP_001275778.1. NC_009523.1.
3D structure databases
ProteinModelPortal	A5UT75.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UT75.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5208384.
GenomeReviews	Gene locus RoseRS_1430 in contig CP000686_GR.
KEGG	rrs:RoseRS_1430.
PATRIC	23350984. VBIRosSp109359_1553.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	IERNIRH.
ProtClustDB	PRK06599.
Enzyme and pathway databases
BioCyc	RSP357808:ROSERS_1430-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5UT75_ROSS1

Accession

Primary (citable) accession number: A5UT75

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)