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Protein

Peptide deformylase

Gene

def

Organism
Roseiflexus sp. (strain RS-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091IronUniRule annotation
Metal bindingi152 – 1521IronUniRule annotation
Active sitei153 – 1531UniRule annotation
Metal bindingi156 – 1561IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciRSP357808:GH5Z-1065-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDFUniRule annotation
Alternative name(s):
Polypeptide deformylaseUniRule annotation
Gene namesi
Name:defUniRule annotation
Ordered Locus Names:RoseRS_1052
OrganismiRoseiflexus sp. (strain RS-1)
Taxonomic identifieri357808 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaChloroflexalesRoseiflexineaeRoseiflexaceaeRoseiflexus
ProteomesiUP000006554: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Peptide deformylasePRO_1000200745Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi357808.RoseRS_1052.

Structurei

3D structure databases

ProteinModelPortaliA5US58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243508.
KOiK01462.
OMAiTIWGEPV.
OrthoDBiEOG664CMF.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

A5US58-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALRRILRID NPDDKKILTT RCHPVRLPNP ALKQLVADMF ETMHAASGVG
60 70 80 90 100
LAAPQIGITQ RLAVISIPPV VEERPDGSKV EVAPEQNFVL INPEIIKASD
110 120 130 140 150
QEDVGLEGCL SLPGWYGEVP RAAWVTVEYT DLNGRRQRIR RATGLLGRAL
160 170 180
QHEIDHLDGI LFTERIRDLS TLKDYSEEMA PTAAE
Length:185
Mass (Da):20,567
Last modified:July 10, 2007 - v1
Checksum:i3AB55F07D0F3F19A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000686 Genomic DNA. Translation: ABQ89461.1.
RefSeqiWP_011955814.1. NC_009523.1.
YP_001275411.1. NC_009523.1.

Genome annotation databases

EnsemblBacteriaiABQ89461; ABQ89461; RoseRS_1052.
GeneIDi5207998.
KEGGirrs:RoseRS_1052.
PATRICi23350153. VBIRosSp109359_1145.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000686 Genomic DNA. Translation: ABQ89461.1.
RefSeqiWP_011955814.1. NC_009523.1.
YP_001275411.1. NC_009523.1.

3D structure databases

ProteinModelPortaliA5US58.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi357808.RoseRS_1052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ89461; ABQ89461; RoseRS_1052.
GeneIDi5207998.
KEGGirrs:RoseRS_1052.
PATRICi23350153. VBIRosSp109359_1145.

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243508.
KOiK01462.
OMAiTIWGEPV.
OrthoDBiEOG664CMF.

Enzyme and pathway databases

BioCyciRSP357808:GH5Z-1065-MONOMER.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RS-1.

Entry informationi

Entry nameiDEF_ROSS1
AccessioniPrimary (citable) accession number: A5US58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 10, 2007
Last modified: January 7, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.