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A5UR90 (A5UR90_ROSS1) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP MF_02003
EC=6.1.1.5 HAMAP MF_02003
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP MF_02003
Gene names
Name:ileS HAMAP MF_02003
Ordered Locus Names:RoseRS_0727
OrganismRoseiflexus sp. (strain RS-1) [Complete proteome] [HAMAP] EMBL ABQ89143.1
Taxonomic identifier357808 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Roseiflexus

Protein attributes

Sequence length1069 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003

Cofactor

Zinc By similarity. HAMAP MF_02003

Subunit structure

Monomer By similarity. HAMAP MF_02003

Subcellular location

Cytoplasm By similarity HAMAP MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily. HAMAP MF_02003

Sequences

Sequence LengthMass (Da)Tools
A5UR90 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 4AB7393EC2243525

FASTA1,069121,068
        10         20         30         40         50         60 
MAFQPVDTRV NFPRLEEQVL DLWQTRRIVE RSLESGDKPF VFYEGPPTAN GRPGIHHAIS 

        70         80         90        100        110        120 
RIFKDVICRY RAMQGYRIIG RREGWDTHGL PVEIEVEKKL GFSGKPDIEK YGVAEFNAQC 

       130        140        150        160        170        180 
RKSVWEYIQD WRQFTRRIAY WVSDDAYITY ENDYIESLWW IFRQLWDRGL LFRDYKVSMH 

       190        200        210        220        230        240 
CPRCGTSLSD HEVSLGFRDD VDDPSVWVKF RVRETNQEAL RGAAFLAWTT TPWTLPANVA 

       250        260        270        280        290        300 
LAVKPDATYV LASFDGERLV LAEALVHTVL GDGVTIEQTF RGADLVGTRY ENLFDGVPAP 

       310        320        330        340        350        360 
GDTVDWSRAY TVVADDFVSL EDGTGIVHIA PAYGDLEIGR KYGLPTLFSV DLSGNVLPEF 

       370        380        390        400        410        420 
ASLGFAGKFF KQADPDITRN LRTRGLLLKS GRVKHSYPFC WRCDTPLLYY AKRSWYIRTT 

       430        440        450        460        470        480 
AMKDRLVANN RLINWVPEHI REGRFGNWLE NNIDWAVSRE RYWGTPIPIW VAEDGYAECI 

       490        500        510        520        530        540 
GSLAELEQKV GRSLKDLDLH RPYVDELTWV DPQHGLMRRI PDVADCWFDS GAMPVAQWHY 

       550        560        570        580        590        600 
PMENAEIYEL AKQADYICEA VDQTRGWFYT LHALSTLLFD RPAFKNVICL GHILDAEGQK 

       610        620        630        640        650        660 
MSKSRGNVVD PWMVMNEHGA DALRWYLFTA APPGSPRRFS AGLVAESLRK FLLTLWNTYA 

       670        680        690        700        710        720 
FFVTYANLDG WSPGNNGVAE HLDPATLTPI DRWALARLNS LVRDVTAAFE AYDVTTAGRA 

       730        740        750        760        770        780 
IEDFVDELSN WYVRRNRRRF WKSEHDRDKQ AAYATLYTCL LTVAKLVAPF IPFVADAIYR 

       790        800        810        820        830        840 
NLVGLEAQEA SPDTPESVHL AAWPVVNEAL LDDRLVDDTE VLLKAVSVGR AARRAAGIKV 

       850        860        870        880        890        900 
RQPLREMWVR APTPAALEGL RRFEAELREE LNVKAVRYLD SATSLVEYRF KPNLRLVGRK 

       910        920        930        940        950        960 
YGKLVPALTE ALRDLTGDAA RAAAQAVEEG RNITLSIEGQ TIELLPEEVL VESSSPQGYA 

       970        980        990       1000       1010       1020 
VAEDGGVLVA LDTTLTPELT MEGLARELVR NIQDARKDAG FAISDRIIVY LGGADGDAEV 

      1030       1040       1050       1060 
EAMLRAWGDY VREETLADDL RLCAPPDHVH TTTLELDGGR TLIIGVSRR

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References

[1]"Complete sequence of Roseiflexus sp. RS-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RS-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000686 Genomic DNA. Translation: ABQ89143.1.
RefSeqYP_001275093.1. NC_009523.1.

3D structure databases

ProteinModelPortalA5UR90.
SMRA5UR90. Positions 8-838.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5UR90.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5207666.
GenomeReviewsGene locus RoseRS_0727 in contig CP000686_GR.
KEGGrrs:RoseRS_0727.
PATRIC23349429. VBIRosSp109359_0789.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHBG577712.
OMAPYITYDN.
ProtClustDBPRK06039.

Enzyme and pathway databases

BioCycRSP357808:ROSERS_0727-MONOMER.

Family and domain databases

HAMAPMF_02003. Ile_tRNA_synth_type2.
[Tree]
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-synt.
IPR023586. Ile-tRNA-synt_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
Gene3DG3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01870.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. IleS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameA5UR90_ROSS1
AccessionPrimary (citable) accession number: A5UR90
Entry history
Integrated into UniProtKB/TrEMBL: July 10, 2007
Last sequence update: July 10, 2007
Last modified: January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info