A5UPW5 (SYN_ROSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	RoseRS_0232

Organism

Roseiflexus sp. (strain RS-1) [Complete proteome] [HAMAP]

Taxonomic identifier

357808 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Chloroflexales › Chloroflexaceae › Roseiflexus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000072531

Sequences

	Sequence		Length	Mass (Da)
	A5UPW5 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 2221572CA75537F6	FASTA	440	49,719
	10 20 30 40 50 60 MSLLPTATIS TIAHHNGEVV TLAGWVVHKT EKGKLVFIRL RDGSGVIQCV VFRNNVTEAT 70 80 90 100 110 120 FAAAQQLTIE SSCRITGAVR ADARAPGGFE LDVNAIEIIQ IAPEYPIQPK EHGVEFLMEH 130 140 150 160 170 180 RHLWIRSSKQ HALLRIRAEI IAAAQEWLND QGFVRFDTPI LTPCAAEGTT NLFATPYFDL 190 200 210 220 230 240 GTAYLGQTGQ LYVEAGMMSF GKVYCFGPTF RAEKSKTRRH LTEFWMIEPE VAFALHEDNL 250 260 270 280 290 300 ALQERFVSAI VQRVLERRAD DLATLERDTK PLERCVPPFP RITYDEALKL IAERHADVEG 310 320 330 340 350 360 CTPLEWGEDL GAPHETLIAS LFDRPVFVER FPSAIKAFYM EPDPQRPEVA LCADLLAPEG 370 380 390 400 410 420 YGEIIGGSQR IHDPALLERR IREYGLNVDD YQWYIDLRRY GSVPHSGFGM GIERATAWIA 430 440 GTHHIRETIP FPRMLYRMYP

References

[1]

"Complete sequence of Roseiflexus sp. RS-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000686 Genomic DNA. Translation: ABQ88668.1.
RefSeq	YP_001274618.1. NC_009523.1.
3D structure databases
ProteinModelPortal	A5UPW5.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UPW5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5207167.
GenomeReviews	Gene locus RoseRS_0232 in contig CP000686_GR.
KEGG	rrs:RoseRS_0232.
PATRIC	23348352. VBIRosSp109359_0254.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	AVDYPIT.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	RSP357808:ROSERS_0232-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ROSS1

Accession

Primary (citable) accession number: A5UPW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program