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Protein

Ribose-phosphate pyrophosphokinase

Gene

prs

Organism
Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of ribose 1,5-bisphosphate. Catalyzes the transfer of pyrophosphoryl group from ATP to ribose-5-phosphate to yield phosphoribosyl diphosphate (PRPP) and AMP.UniRule annotation

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase (prs)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92ATPUniRule annotation1
Metal bindingi123MagnesiumUniRule annotation1
Metal bindingi125MagnesiumUniRule annotation1
Binding sitei125ATPUniRule annotation1
Binding sitei164Ribose-5-phosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi33 – 35ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00087; UER00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinaseUniRule annotation (EC:2.7.6.1UniRule annotation)
Short name:
RPPKUniRule annotation
Alternative name(s):
5-phospho-D-ribosyl alpha-1-diphosphateUniRule annotation
Phosphoribosyl diphosphate synthaseUniRule annotation
Phosphoribosyl pyrophosphate synthaseUniRule annotation
Short name:
P-Rib-PP synthaseUniRule annotation
Short name:
PRPP synthaseUniRule annotation
Short name:
PRPPaseUniRule annotation
Gene namesi
Name:prsUniRule annotation
Ordered Locus Names:Msm_1577
OrganismiMethanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)
Taxonomic identifieri420247 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobrevibacter
Proteomesi
  • UP000001992 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000612001 – 298Ribose-phosphate pyrophosphokinaseAdd BLAST298

Interactioni

Protein-protein interaction databases

STRINGi420247.Msm_1577.

Structurei

3D structure databases

ProteinModelPortaliA5UNK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni187 – 189Ribose-5-phosphate bindingUniRule annotation3
Regioni224 – 232Ribose-5-phosphate bindingUniRule annotation9

Sequence similaritiesi

Belongs to the ribose-phosphate pyrophosphokinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00067. Archaea.
COG0462. LUCA.
HOGENOMiHOG000210450.
KOiK00948.
OMAiITINPHE.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_A. RibP_PPkinase_A. 1 hit.
InterProiIPR029099. Pribosyltran_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 2 hits.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.

Sequencei

Sequence statusi: Complete.

A5UNK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIGGSASQD LAAHVAEELN DDLCYVETKK FPDGERYLRV DGEIDDEVTV
60 70 80 90 100
IQSTGYPQDE NLMELLFLIS NLKDLGAKKV RVVVPYMGYA RQEKRFHDGE
110 120 130 140 150
AVSAKIVANL IQSAGADEFI TFNIHEKCVL DFFDIPTTNI SAMGAIAEYI
160 170 180 190 200
DEKIDEKPLI VAPDKGAYPF AQEIAEILDC ECTYLSKVRL GPDKVETRIV
210 220 230 240 250
DVEGSSDETV NVDSVKGKKA FIIDDIIATG GTIVNAVKIL KEYGAKSVDV
260 270 280 290
CCVHPILVNG ATMRIYAAGA NSLISTNSLS ADSSRVSLAK SIANVLRD
Length:298
Mass (Da):32,403
Last modified:July 10, 2007 - v1
Checksum:iC8145C41AB7D1E39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000678 Genomic DNA. Translation: ABQ87782.1.
RefSeqiWP_004033541.1. NC_009515.1.

Genome annotation databases

EnsemblBacteriaiABQ87782; ABQ87782; Msm_1577.
GeneIDi5216111.
KEGGimsi:Msm_1577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000678 Genomic DNA. Translation: ABQ87782.1.
RefSeqiWP_004033541.1. NC_009515.1.

3D structure databases

ProteinModelPortaliA5UNK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi420247.Msm_1577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ87782; ABQ87782; Msm_1577.
GeneIDi5216111.
KEGGimsi:Msm_1577.

Phylogenomic databases

eggNOGiarCOG00067. Archaea.
COG0462. LUCA.
HOGENOMiHOG000210450.
KOiK00948.
OMAiITINPHE.

Enzyme and pathway databases

UniPathwayiUPA00087; UER00172.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 2 hits.
HAMAPiMF_00583_A. RibP_PPkinase_A. 1 hit.
InterProiIPR029099. Pribosyltran_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 2 hits.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKPRS_METS3
AccessioniPrimary (citable) accession number: A5UNK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.