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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSMI420247:GHWZ-1490-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Msm_1452
OrganismiMethanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861)
Taxonomic identifieri420247 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobrevibacter
ProteomesiUP000001992 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 555555Glutamate--tRNA ligasePRO_0000331003Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi420247.Msm_1452.

Structurei

3D structure databases

ProteinModelPortaliA5UN79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi103 – 11311"HIGH" regionAdd
BLAST

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiMRFAPNP.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5UN79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDLEEIVYK HALLNAAKHK GSANPGAVIG SIMSQEPDLR SRAKEIGPIA
60 70 80 90 100
GKIVAQVNKL SEDEQSAQMA KYHVEVKENK QKKEEGLQEL PGSHDNVVMR
110 120 130 140 150
FAPNPSGPLH IGHARAAVPN AEYVKRYGGK LILRIEDTDP KRVFEPAYDL
160 170 180 190 200
IPQDLEWLGI KADEVYYQSD RFEIYYDYAR QLIEKGAAYM CTCDGATFKE
210 220 230 240 250
LKDNCKPCPC RDNSVEKNLE LWDKFDQMHA GEAVLRVKTD INHKNPAIRD
260 270 280 290 300
WVAMRIVEET HPRLGNKYRV YPMMNFSVAV DDHLMGMSHV LRGKDHLANS
310 320 330 340 350
EKQKYLYDHM GWDVPEFIHY GRLKMEDIAL STSKALEGIS SGKYSGWDDP
360 370 380 390 400
RLGTLKAIAR RGIQPQTIYN LITEIGVKMS DSAISWKKIY GLNRNFLEPI
410 420 430 440 450
ANRYFFVENP VEITVDGYED GAVDIERPLH ADHEDRGNRI LPFAGKAYLA
460 470 480 490 500
SEDVKDGISR LMDAVNVDID GDKITYNSTS FEQARDLKAK IIQWVPVEDN
510 520 530 540 550
VNVSIVMDDA STKTGLGEGA LKDLKVGDVV QFERVGFARL DEIKDNELVF

YYAHK
Length:555
Mass (Da):62,827
Last modified:July 10, 2007 - v1
Checksum:i49F61D8B7532ECDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000678 Genomic DNA. Translation: ABQ87657.1.
RefSeqiWP_004035429.1. NC_009515.1.
YP_001274025.1. NC_009515.1.

Genome annotation databases

EnsemblBacteriaiABQ87657; ABQ87657; Msm_1452.
GeneIDi5216855.
KEGGimsi:Msm_1452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000678 Genomic DNA. Translation: ABQ87657.1.
RefSeqiWP_004035429.1. NC_009515.1.
YP_001274025.1. NC_009515.1.

3D structure databases

ProteinModelPortaliA5UN79.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi420247.Msm_1452.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ87657; ABQ87657; Msm_1452.
GeneIDi5216855.
KEGGimsi:Msm_1452.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000073585.
KOiK01885.
OMAiMRFAPNP.

Enzyme and pathway databases

BioCyciMSMI420247:GHWZ-1490-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_A. Glu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004526. Glu-tRNA-synth_arc/euk.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR022888. Glu_tRNA_synth_arc.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00463. gltX_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PS / ATCC 35061 / DSM 861.

Entry informationi

Entry nameiSYE_METS3
AccessioniPrimary (citable) accession number: A5UN79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 10, 2007
Last modified: July 22, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.