A5UN11 (A5UN11_METS3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit HAMAP MF_00324
Short name=Pol II HAMAP MF_00324
EC=2.7.7.7 HAMAP MF_00324

Gene names

Name:	polC HAMAP MF_00324
Ordered Locus Names:	Msm_1384

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP] EMBL ABQ87589.1

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	1101 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324 Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity. HAMAP MF_00324
Sequence similarities	Belongs to the archaeal DNA polymerase II family. HAMAP MF_00324

Ontologies

Keywords
Biological process	DNA replication HAMAP MF_00324
Ligand	DNA-binding HAMAP MF_00324
Molecular function	DNA-directed DNA polymerase HAMAP MF_00324 Exonuclease HAMAP MF_00324 Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme HAMAP MF_00324
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A5UN11 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 2075BA23AC798998	FASTA	1,101	123,509
	10 20 30 40 50 60 MEINMDYFDR LEADTKALYE IANEARSKGL DVETKSEVPL AKDLAERVEG LVGPEGVAKR 70 80 90 100 110 120 IKELEQDITR EEVAFEIAAE IASGKFELTK EKANYNEEQR CDQGLRTALA ILTEGVVAAP 130 140 150 160 170 180 LEGISQVKIK ENFDSTKYIA VYFAGPIRSA GGTAAALAVL LGDKIKEAIG IDDFKPIDDE 190 200 210 220 230 240 IERYVEEVEL YESEVTNLQY SPTPEEVRFA ANHIPVEVTG EQTDQVEVSH RDLERVETNN 250 260 270 280 290 300 IRGGALLAMV EGVIQKSKKI LKISKKLKLD SWGWLSEYSK PKSDDKKSDD DSTDLVTEPK 310 320 330 340 350 360 YIQDIIGGRP VLGYPSEKGG FRLRYGRSRN TGLATMGVHP ATMALLDFLA VGTQLKIEYP 370 380 390 400 410 420 GKGNCVVPVD SIEGPIVKLK NGDVVKIDKI KQAKELKNQV VEILFLGDML VAFGEFLRNN 430 440 450 460 470 480 QPLMPSGWCE EWWLGLLQKS DKFDSTDETL DLHRLEYDYI PATEAFKLSE EYDIPLHPKY 490 500 510 520 530 540 TYCYNDATVN DLNSLIDVIL ENKSKYSPEN GLTIDLDYRK RVLEIIGVPH TVKDGKITID 550 560 570 580 590 600 KDHSYALINT LSDNIPVKEH TLEALNEVSP IEIKNKAPAY IGTRVGRPEK SKERLMRPAP 610 620 630 640 650 660 HGLFPIGNYG GSRRLVATAA KKGNVKVDLA RRKCTQCKIS SFQSICPHCG APTEISPHSV 670 680 690 700 710 720 KNINLSAMLK KASDNVKVRK VDEMKGVVGM ISESKLPEPL EKGILRSKNE VFTFKDGTIR 730 740 750 760 770 780 HDSTDLPLTH FIPKEIGVTV EKLLEMGYTK DCYGNPIEND EQIIELRVQD IVISNNCADY 790 800 810 820 830 840 LVRTANFIDD ELERYYGMER FYNVKDKSDL IGHLVAGLAP HTSAGVLGRI VGFTKALCCY 850 860 870 880 890 900 AHPYFHSAKR RNCDSDEDAV MLLLDALINF SKSYLPNTRG GSMDAPLVLS SRIDPEEIDD 910 920 930 940 950 960 ESHNLDIFER FPVEFYEKTY EPLKPAEVLE YIDNVEMHLG TPQQYEGLMF SHHTSSIHAG 970 980 990 1000 1010 1020 PTVCLYKRLP SMREKVEAQI ALAENIRAVD QRGVVEKVLS SHFLPDIMGN SRAFSKQKVR 1030 1040 1050 1060 1070 1080 CTKCGSKYRR MPLTGKCKCG GNLILSVSKG SVTKYLEISQ DLVNRYPVSH YLRQRLEIQE 1090 1100 FGIHSLFESD KSKQSSLDVF F

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000678 Genomic DNA. Translation: ABQ87589.1.
RefSeq	YP_001273957.1. NC_009515.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5UN11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5217195.
GenomeReviews	Gene locus Msm_1384 in contig CP000678_GR.
KEGG	msi:Msm_1384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04008.
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK04023.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5UN11_METS3

Accession

Primary (citable) accession number: A5UN11

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)