A5UMW9 (PURL_METS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Msm_1342

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP]

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050321
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A5UMW9 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 7B5C15DACD13162D	FASTA	716	77,739
	10 20 30 40 50 60 MTLTDSEMEY IEGILGRKMN ELEEGMLDVM FSEHCSYKSS RPILGTFPTE GENIILGPGD 70 80 90 100 110 120 DAGLVSVTDK YALAVGMESH NHPSAIEPYG GAGTGIGGIL RDIISMGARP IALLDSLRFG 130 140 150 160 170 180 SLDDEKSKYL FEHVVEGISD YGNRVGVPTV AGEVEFDDSF RTNPLVNVMC VGLVEKDKIV 190 200 210 220 230 240 RGKAPYVGDV FLLMGGTTGR DGIHGVTFAS EELTSDSETE DRPAVQVADP FTKKRVLEAS 250 260 270 280 290 300 LEILDEINVS GVKDLGGGGL TCCISELVDE SNNGARVDLR NIPLRETGMT PYEIMLSESQ 310 320 330 340 350 360 ERMVFVINPK DVKKAQEICD KHEIVSAVIG EVIDGNNMII DDEGSSLADL PTILLADPPS 370 380 390 400 410 420 LNRELKEIAE DTSKVEVDHP PVRQSLLELL SSPNIASKQW VYKQYDHEVQ VRTVVKPGDD 430 440 450 460 470 480 AAVLRIDDDT AIALTTDANT IHTKLSPFDG GAGCVAEAIR NVISMGATPY AVVDCLNFGN 490 500 510 520 530 540 PETPEILWQF KRTIEGMSLV AENFNAPVIS GNVSFYNETE GIKINPTPAV GVIGVENIEN 550 560 570 580 590 600 IRTMDFKNSG DKILLIGTTY DEVTGSEYHR TIHNIEKGMA PRIRIEDELA NGKTILKLLD 610 620 630 640 650 660 EDSSKNITAV HDVSHGGLAV ALSEMVMKGN IGCEIDLDSI ITSEDLEESD LIYSESHGRY 670 680 690 700 710 IITVNADAVD EILNKIDVPV AIIGEVKGAV LKLGDNEFTI DELNDSYHGV IEKYMA

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PS / ATCC 35061 / DSM 861.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000678 Genomic DNA. Translation: ABQ87547.1.
RefSeq	YP_001273915.1. NC_009515.1.
3D structure databases
ProteinModelPortal	A5UMW9.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UMW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5217249.
GenomeReviews	Gene locus Msm_1342 in contig CP000678_GR.
KEGG	msi:Msm_1342.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04101.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_METS3

Accession

Primary (citable) accession number: A5UMW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program