A5UMK8 (SYR_METS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Msm_1231

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP]

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter › Methanobrevibacter smithii

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_1000018065
Regions
	Motif	131 – 141	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	A5UMK8 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: AA478897DDF6F514	FASTA	575	65,338
	10 20 30 40 50 60 MYFEIEKQAI DAISDALDKF EVDNTLENFQ VEDEKNFRLE FPPNPDMGDL ASTIAFSLAK 70 80 90 100 110 120 KLRKAPNLIA SEIVEKLEIP EIFEKVEAIG PYVNFFIDYS NFSKKLLEYV GKDYGHLPKA 130 140 150 160 170 180 DEKIILEHTS ANPNGPLHIG HVRNSIFGDS LNRLLKVAGR EVETQYYVND MGRQIAIIVF 190 200 210 220 230 240 GITELGLKIE DQEGDKIDHK IGRLYFKANQ KLNEDESLVS HVDNLIERYE GGAEPELNKI 250 260 270 280 290 300 FEEVVESCLL GIKETLHRIN INHDDFVWEG QFVRSGEVDD MIKYFDHEGF VSYGDVTYID 310 320 330 340 350 360 LTCFQIEKEF VLRRSDGTSL YSTRDLAYHR YKATQGDVVL DILGSDHKLA AQQINVIFKE 370 380 390 400 410 420 ILREIPPEVI FYEFITLPSG SMSTRKGVFV SVDELVDEAV KRAADEIKSR NPDLTDEEIK 430 440 450 460 470 480 PMAEDIGVGA IRFFIAKLSP EKHLTFKWDE ALSFERGCAS IQYAHARACK LLKKSGKDVS 490 500 510 520 530 540 SLAVSDDWVP NENEKDLIRT IAKFPQVIED CANKKRIHNI TQYCQDLAGA FNKFYKAEQV 550 560 570 IGSDVEDTRL VLVDRAKTTL KNALDILGVP APQKM

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PS / ATCC 35061 / DSM 861.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000678 Genomic DNA. Translation: ABQ87436.1.
RefSeq	YP_001273804.1. NC_009515.1.
3D structure databases
ProteinModelPortal	A5UMK8.
ModBase	Search...
Protein-protein interaction databases
STRING	420247.Msm_1231.
Proteomic databases
PRIDE	A5UMK8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ87436; ABQ87436; Msm_1231.
GeneID	5216015.
KEGG	msi:Msm_1231.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247213.
KO	K01887.
OMA	HTSANPN.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	MSMI420247:GHWZ-1272-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_METS3

Accession

Primary (citable) accession number: A5UMK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program