Reviewed, UniProtKB/Swiss-Prot A5UMK4 (PUR2_METS3)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	Msm_1227

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP]

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_1000018828
Regions
	Domain	106 – 318	213	ATP-grasp
	Nucleotide binding	133 – 196	64	ATP By similarity
Sites
	Metal binding	276	1	Magnesium or manganese 1 By similarity
	Metal binding	288	1	Magnesium or manganese 1 By similarity
	Metal binding	288	1	Magnesium or manganese 2 By similarity
	Metal binding	290	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5UMK4-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: FE37BFFD1F9487F9	FASTA	436	47,267
	10 20 30 40 50 60 MKVLVVGTGA REHAIADALK DDVELYCYMS KVNPGISKIA EFAQGDEGEI EKVAKFAVDN 70 80 90 100 110 120 NIDIAFIGPE APLGKGIVDE LEKNGISCVG PSQSAARIET DKSFMRKLFE DYDIEGSLVY 130 140 150 160 170 180 KVFDNYDDVS AFLDDFDRDV VVKPVGLTGG KGVKIVGDHL KDNQEAKEYS KEVIDNAMGG 190 200 210 220 230 240 FTQVIIEERL IGEEFTIQAF CDGTHLAPMP AAQDHPHAFE GDVGAITGGM GSYSDKGGLL 250 260 270 280 290 300 PFLSQDDYDE AVKIMEATLK AIAKEAEPYK GILYGQFMLT ADGPKLIEYN ARFGDPEAMN 310 320 330 340 350 360 VLPLLKTPLA DVCQAIVDGN LDKVEFNDKA SVCKYIVPDG YPETSHAGET IEVDEKTIED 370 380 390 400 410 420 LGAKVFYAAV GLEDDEIHLS GSRALGIVAS GDSIEEAEKI AEKACACIKG NVYHRSDVGT 430 TDLVNKRVEH MKEILN

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000678 Genomic DNA. Translation: ABQ87432.1.
RefSeq	YP_001273800.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5UMK4.
Genome annotation databases
GeneID	5216011.
GenomeReviews	Gene locus Msm_1227 in contig CP000678_GR.
KEGG	msi:Msm_1227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IEYNCRF.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_METS3

Accession

Primary (citable) accession number: A5UMK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)