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A5UMK4 (PUR2_METS3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Msm_1227
OrganismMethanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP]
Taxonomic identifier420247 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobrevibacter

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 436436Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000018828

Regions

Domain106 – 318213ATP-grasp
Nucleotide binding133 – 19664ATP By similarity

Sites

Metal binding2761Magnesium or manganese 1 By similarity
Metal binding2881Magnesium or manganese 1 By similarity
Metal binding2881Magnesium or manganese 2 By similarity
Metal binding2901Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5UMK4 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: FE37BFFD1F9487F9

FASTA43647,267
        10         20         30         40         50         60 
MKVLVVGTGA REHAIADALK DDVELYCYMS KVNPGISKIA EFAQGDEGEI EKVAKFAVDN 

        70         80         90        100        110        120 
NIDIAFIGPE APLGKGIVDE LEKNGISCVG PSQSAARIET DKSFMRKLFE DYDIEGSLVY 

       130        140        150        160        170        180 
KVFDNYDDVS AFLDDFDRDV VVKPVGLTGG KGVKIVGDHL KDNQEAKEYS KEVIDNAMGG 

       190        200        210        220        230        240 
FTQVIIEERL IGEEFTIQAF CDGTHLAPMP AAQDHPHAFE GDVGAITGGM GSYSDKGGLL 

       250        260        270        280        290        300 
PFLSQDDYDE AVKIMEATLK AIAKEAEPYK GILYGQFMLT ADGPKLIEYN ARFGDPEAMN 

       310        320        330        340        350        360 
VLPLLKTPLA DVCQAIVDGN LDKVEFNDKA SVCKYIVPDG YPETSHAGET IEVDEKTIED 

       370        380        390        400        410        420 
LGAKVFYAAV GLEDDEIHLS GSRALGIVAS GDSIEEAEKI AEKACACIKG NVYHRSDVGT 

       430 
TDLVNKRVEH MKEILN 

« Hide

References

[1]"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut."
Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I.
Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PS / ATCC 35061 / DSM 861.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000678 Genomic DNA. Translation: ABQ87432.1.
RefSeqYP_001273800.1. NC_009515.1.

3D structure databases

ProteinModelPortalA5UMK4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING420247.Msm_1227.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ87432; ABQ87432; Msm_1227.
GeneID5216011.
KEGGmsi:Msm_1227.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAYKGFLYA.

Enzyme and pathway databases

BioCycMSMI420247:GHWZ-1263-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_METS3
AccessionPrimary (citable) accession number: A5UMK4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways