A5UMJ9 (ILVC_METS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Msm_1222

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP]

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000050534
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A5UMJ9 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 5147E15535D6573C	FASTA	330	36,106
	10 20 30 40 50 60 MKMYYESDVN TDALEGKTVA VMGYGSQGRA QSRNMADSGV NVIVGLRENG NSWNLAKEDG 70 80 90 100 110 120 MTVKTFSDAA KEADIIHILL PDEIQEKVYN EQIAPYVEAG NTISFSHGYN IHFGLIKPDE 130 140 150 160 170 180 DVNIVMFAPK GPGSRVRTTY LDGFGIPGLV AIEQDATGDA LQLALGMAKA CGFTKAGVIE 190 200 210 220 230 240 TTFKEETETD LFGEQAVLCG GLTALINAGF QTLVEAGYQP EIAYFETCHE VKLIVDDIYE 250 260 270 280 290 300 HGFGGMWKDV SNTAEYGGLT RRDYVITEET KKGMKKVLSE IQDGTFKKQF ADENATDAAN 310 320 330 LKEMRAAEDQ ETIEVVGERL RKACGLQKDD

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PS / ATCC 35061 / DSM 861.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000678 Genomic DNA. Translation: ABQ87427.1.
RefSeq	YP_001273795.1. NC_009515.1.
3D structure databases
ProteinModelPortal	A5UMJ9.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UMJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5216052.
GenomeReviews	Gene locus Msm_1222 in contig CP000678_GR.
KEGG	msi:Msm_1222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04335.
HOGENOM	HBG286525.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_METS3

Accession

Primary (citable) accession number: A5UMJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program