A5UMI1 (MTD_METS3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
F420-dependent methylenetetrahydromethanopterin dehydrogenase
Short name=MTD
EC=1.5.99.9

Alternative name(s):
Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase

Gene names

Name:	mtd
Ordered Locus Names:	Msm_1204

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP]

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reduction of methenyl-H4MPT+ to methylene-H4MPT By similarity. HAMAP MF_00058
Catalytic activity	5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_00058
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1. HAMAP MF_00058
Sequence similarities	Belongs to the MTD family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro methylenetetrahydromethanopterin dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	F420-dependent methylenetetrahydromethanopterin dehydrogenase HAMAP MF_00058		PRO_1000117814

Sequences

	Sequence		Length	Mass (Da)
	A5UMI1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 44A26F8351D6EEDD	FASTA	275	29,231
	10 20 30 40 50 60 MVVKIGIIKS GNIGTSPVLD LLLDERADRP NIDVRVFGSG AKMNPEQVED VVPKVDQFDP 70 80 90 100 110 120 DFCIFISPNP GAPGPAKARE LLSEKDIPAI IIGDAPGKGK KDEMDEQGLG YIIVMSDPMI 130 140 150 160 170 180 GAKREWLDPT EMAIFNADIL KVLAETGALR LVQNTIDGVI DGAAAGNIEL PKLIITAEKA 190 200 210 220 230 240 VEAAGFENPY AKAKAIAAYE MAGAVANLDM KGCFMTKGFE NFIPLVAAAH EMAASAAALA 250 260 270 DEAREIEKGN DSVLRTPHMK EGNTGCKTDL ISKPE

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PS / ATCC 35061 / DSM 861.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000678 Genomic DNA. Translation: ABQ87409.1.
RefSeq	YP_001273777.1. NC_009515.1.
3D structure databases
ProteinModelPortal	A5UMI1.
SMR	A5UMI1. Positions 1-275.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UMI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5217296.
GenomeReviews	Gene locus Msm_1204 in contig CP000678_GR.
KEGG	msi:Msm_1204.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04866.
HOGENOM	HBG538567.
OMA	NPYAKAK.
ProtClustDB	PRK00994.
Family and domain databases
HAMAP	MF_00058. MTD. [Tree]
InterPro	IPR002844. Methylene_DH. [Graphical view]
KO	K00319.
Pfam	PF01993. MTD. 1 hit. [Graphical view]
PIRSF	PIRSF005627. MTD. 1 hit.
ProDom	PD017604. Methylene_DH. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF102324. MeTD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTD_METS3

Accession

Primary (citable) accession number: A5UMI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program