A5UME8 (A5UME8_METS3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
EC=6.3.1.5 HAMAP MF_00193

Gene names

Name:	nadE HAMAP MF_00193
Ordered Locus Names:	Msm_1171

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP] EMBL ABQ87376.1

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family. HAMAP MF_00193

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_00193 NAD HAMAP MF_00193 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00193 EMBL ABQ87376.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	35 – 42	8	ATP By similarity HAMAP MF_00193
Sites
	Active site	37	1	By similarity HAMAP MF_00193

Sequences

	Sequence		Length	Mass (Da)
	A5UME8 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: C1EEF1CDD049947E	FASTA	266	29,753
	10 20 30 40 50 60 MNELPKLDTK TTKESIIKFI QDKVSEANAK GLVVGLSGGI DSTLTAYLAT EAVGKENVFG 70 80 90 100 110 120 IVMPSTTTPT EDKIHGTDIA KILDIDYKEM AIDSVLNEFL YITQYKTENE QLAIGNLKAR 130 140 150 160 170 180 IRMSIIYYYA NSKGYLVSGT GNKSEILIGY FTKHGDGACD IEPIGDLYKT DVFELAKYMG 190 200 210 220 230 240 VPEEIINKPP RAGLWNNQTD EDEIGMTYEN LDKILYQYND KETSKDEIAK NLDISIDEID 250 260 MIINKVKRNA HKSKVPESPE KTVMVI

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000678 Genomic DNA. Translation: ABQ87376.1.
RefSeq	YP_001273744.1. NC_009515.1.
3D structure databases
ProteinModelPortal	A5UME8.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UME8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5217405.
GenomeReviews	Gene locus Msm_1171 in contig CP000678_GR.
KEGG	msi:Msm_1171.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	DGAVDCH.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5UME8_METS3

Accession

Primary (citable) accession number: A5UME8

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)