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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111NAD; via amide nitrogenUniRule annotation1
Active sitei141NucleophileUniRule annotation1
Binding sitei169NADUniRule annotation1
Binding sitei302NAD; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation (EC:1.2.1.59UniRule annotation)
Short name:
GAPDHUniRule annotation
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gapUniRule annotation
Ordered Locus Names:Msm_0962
OrganismiMethanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)
Taxonomic identifieri420247 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanobrevibacter
Proteomesi
  • UP000001992 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003009711 – 338Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST338

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi420247.Msm_0962.

Structurei

3D structure databases

ProteinModelPortaliA5ULT9.
SMRiA5ULT9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 142Glyceraldehyde 3-phosphate bindingUniRule annotation3
Regioni195 – 196Glyceraldehyde 3-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00493. Archaea.
COG0057. LUCA.
HOGENOMiHOG000223361.
KOiK00150.
OMAiNAIVPNP.
OrthoDBiPOG093Z05Z6.

Family and domain databases

HAMAPiMF_00559. G3P_dehdrog_arch. 1 hit.
InterProiView protein in InterPro
IPR000846. DapB_N.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR10836. PTHR10836. 1 hit.
PTHR10836:SF76. PTHR10836:SF76. 1 hit.
PfamiView protein in Pfam
PF01113. DapB_N. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PIRSFiPIRSF000149. GAP_DH. 1 hit.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

A5ULT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSVAINGYG TIGKRVADAV AAQDDMKVIG VSKTRPNYES RTAVEEKGYD
60 70 80 90 100
LYIGIPERAD QFKEAGIEIA GTVEDMIQEA DVVVDCTPGT IGPQNLEMYK
110 120 130 140 150
KAGVKAIYQG GEDHELTGLS FNAISNYDDS YGKDYTRVVS CNTTGLTRTL
160 170 180 190 200
STIDPIADIK KVRAVMVRRG SDPSEVKKGP INSIVPNPPK VPSHHGPDVK
210 220 230 240 250
TVMEGIDVTT MALLVPTTLM HQHNIMVEIN NEVETQEIID ALEKRSRVLV
260 270 280 290 300
VDASEGLGST AELMEYAKEL GRNRNDLYEI PVWRESINVV GNELYYMQAV
310 320 330
HQESDVVPEN IDAIRALLEM ESDNEKSIAK TNKAMGIL
Length:338
Mass (Da):37,130
Last modified:July 10, 2007 - v1
Checksum:iC534794D04B1BA6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000678 Genomic DNA. Translation: ABQ87167.1.
RefSeqiWP_004032973.1. NC_009515.1.

Genome annotation databases

EnsemblBacteriaiABQ87167; ABQ87167; Msm_0962.
GeneIDi5217242.
KEGGimsi:Msm_0962.
PATRICifig|420247.28.peg.959.

Similar proteinsi

Entry informationi

Entry nameiG3P_METS3
AccessioniPrimary (citable) accession number: A5ULT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 10, 2007
Last modified: November 22, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families