A5ULE8 (A5ULE8_METS3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase HAMAP MF_01208
Short name=OPRT HAMAP MF_01208
Short name=OPRTase HAMAP MF_01208
EC=2.4.2.10 HAMAP MF_01208

Gene names

Name:	pyrE HAMAP MF_01208
Ordered Locus Names:	Msm_0821

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP] EMBL ABQ87026.1

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily. HAMAP MF_01208

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis HAMAP MF_01208
Ligand	Magnesium HAMAP MF_01208
Molecular function	Glycosyltransferase HAMAP MF_01208 EMBL ABQ87026.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	117 – 125	9	5-phosphoribose 1-diphosphate binding By similarity HAMAP MF_01208
Sites
	Binding site	91	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	92	1	5-phosphoribose 1-diphosphate By similarity HAMAP MF_01208
	Binding site	95	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity HAMAP MF_01208
	Binding site	121	1	Orotate By similarity HAMAP MF_01208
	Binding site	149	1	Orotate By similarity HAMAP MF_01208

Sequences

	Sequence		Length	Mass (Da)
	A5ULE8 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: FCCDF724B07BC949	FASTA	174	18,811
	10 20 30 40 50 60 MITKEYLINL LKENGVFLKG DFTLSSGKKS DYYINMKKAI TDPEILSTIA KLITKSISED 70 80 90 100 110 120 NIDKVAGPAL GAVPIATAVS LESEIPLLMI RKEKKGYGTS KLIEGELNEG DDVIVVEDVT 130 140 150 160 170 TTGGSLLKAI KAIQDNGGSV KRAFVVVDRQ EGAIEAFDSE GVKLEPLITV DEFF

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000678 Genomic DNA. Translation: ABQ87026.1.
RefSeq	YP_001273394.1. NC_009515.1.
3D structure databases
ProteinModelPortal	A5ULE8.
ModBase	Search...
Protein-protein interaction databases
STRING	A5ULE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5216222.
GenomeReviews	Gene locus Msm_0821 in contig CP000678_GR.
KEGG	msi:Msm_0821.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG09150.
HOGENOM	HBG404341.
OMA	GKEADYY.
ProtClustDB	PRK00455.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5ULE8_METS3

Accession

Primary (citable) accession number: A5ULE8

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)