A5UKE1 (A5UKE1_METS3) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase HAMAP MF_01265
EC=1.4.1.21 HAMAP MF_01265

Gene names

Name:	nadX HAMAP MF_01265
Ordered Locus Names:	Msm_0464

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP] EMBL ABQ86669.1

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family. HAMAP MF_01265

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis HAMAP MF_01265
Ligand	NAD HAMAP MF_01265 NADP HAMAP MF_01265
Molecular function	Oxidoreductase HAMAP MF_01265 EMBL ABQ86669.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	205	1	By similarity HAMAP MF_01265
	Binding site	121	1	NAD; via amide nitrogen By similarity HAMAP MF_01265
	Binding site	177	1	NAD By similarity HAMAP MF_01265

Sequences

	Sequence		Length	Mass (Da)
	A5UKE1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 9E0E6A753976DC07	FASTA	254	27,445
	10 20 30 40 50 60 MKVGIIGCGA IANIIASTIV PEDNNIDIEY FYDNDVERAE NLASFANGVA VLDLNDMLDK 70 80 90 100 110 120 VDLVLECASP DSVKEHAPNV LSHGIDMIIM SIGAFMDKEF YSKVNDICKK KGTRIHLPSG 130 140 150 160 170 180 AVVGLDGIKA VADFNLEEVN LVTRKSPKSL GKDIDEEEVL FEGKASEAVK EFPLNINVAA 190 200 210 220 230 240 TISLACNQDI NVKIIVDPNV DRNVHEITAK GDFGEFKTTT KNFPCSANPK TSMLAALSAI 250 KLLKSFNESI TVGL

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000678 Genomic DNA. Translation: ABQ86669.1.
RefSeq	YP_001273037.1. NC_009515.1.
3D structure databases
ProteinModelPortal	A5UKE1.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UKE1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5217131.
GenomeReviews	Gene locus Msm_0464 in contig CP000678_GR.
KEGG	msi:Msm_0464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG05209.
HOGENOM	HBG649642.
OMA	ECAGHSA.
ProtClustDB	PRK13304.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR022487. Asp_DH_NAD_synth_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K06989.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMs	TIGR03855. NAD_NadX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5UKE1_METS3

Accession

Primary (citable) accession number: A5UKE1

Entry history

Integrated into UniProtKB/TrEMBL:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	November 16, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)