Reviewed, UniProtKB/Swiss-Prot A5UK20 (GUAAA_METS3)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	Msm_0343

Organism

Methanobrevibacter smithii (strain PS / ATCC 35061 / DSM 861) [Complete proteome] [HAMAP]

Taxonomic identifier

420247 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanobrevibacter

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000300235
Regions
	Domain	2 – 189	188	Glutamine amidotransferase type-1
Sites
	Active site	76	1	Nucleophile By similarity
	Active site	163	1	By similarity
	Active site	165	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5UK20-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 9B30ACC7F65697A1	FASTA	190	21,329
	10 20 30 40 50 60 MTILVINNKG QYNHRIQRSL QYLKIPTELV PNTLSIEEIE AKNPIGLILG GGPSIEGAGN 70 80 90 100 110 120 SEEYIKHFDI PILGICLGHQ LIAKAYGGQI DTSNTESYAK VEINIVNDEN LFSGLAPKME 130 140 150 160 170 180 VWSSHKDEVK SIPDDFEILA NSNLCDVESF KHTEKDVYGI QFHPEVHHTP KGSTIFENFY 190 EICKKRCNND

References

[1]

"Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut." Samuel B.S., Hansen E.E., Manchester J.K., Coutinho P.M., Henrissat B., Fulton R., Latreille P., Kim K., Wilson R.K., Gordon J.I. Proc. Natl. Acad. Sci. U.S.A. 104:10643-10648(2007) [PubMed: 17563350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000678 Genomic DNA. Translation: ABQ86548.1.
RefSeq	YP_001272916.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5UK20.
Genome annotation databases
GeneID	5216696.
GenomeReviews	Gene locus Msm_0343 in contig CP000678_GR.
KEGG	msi:Msm_0343.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GQYVHRI.
Family and domain databases
HAMAP	MF_01510. [Tree]
InterPro	IPR006220. Anth_synthII. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR004739. GMP_synth_N. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00096. GATASE.
TIGRFAMs	TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_METS3

Accession

Primary (citable) accession number: A5UK20

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 10, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)