Reviewed, UniProtKB/Swiss-Prot A5UI29 (DSBD_HAEIG)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbD
    EC=1.8.1.8
Alternative name(s):
    Protein-disulfide reductase
      Short name=Disulfide reductase

Gene names

Name:	dsbD
Ordered Locus Names:	CGSHiGG_07975

Organism

Haemophilus influenzae (strain PittGG) [Complete proteome] [HAMAP]

Taxonomic identifier

374931 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity.
Catalytic activity	Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbD subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Redox-active center Signal Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP protein-disulfide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 579	563	Thiol:disulfide interchange protein dsbD HAMAP MF_00399		PRO_1000049610
Regions
	Transmembrane	178 – 198	21	Potential
	Transmembrane	230 – 250	21	Potential
	Transmembrane	254 – 274	21	Potential
	Transmembrane	296 – 316	21	Potential
	Transmembrane	337 – 357	21	Potential
	Transmembrane	376 – 396	21	Potential
	Transmembrane	397 – 417	21	Potential
	Transmembrane	420 – 440	21	Potential
	Domain	449 – 579	131	Thioredoxin
Amino acid modifications
	Disulfide bond	124 ↔ 129		Redox-active By similarity
	Disulfide bond	193 ↔ 315		Redox-active By similarity
	Disulfide bond	495 ↔ 498		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5UI29-1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: A156D89A9059E681	FASTA	579	64,454
	10 20 30 40 50 60 MKKLFLFFTL IFTAFAANSG LFDKKQTFLK VDDAFAFSAT LSTDKSQLQA HWDIADGYYL 70 80 90 100 110 120 YQDKISAELV GKSNPLSLHT QQAAELHQDP YFGEVKVFTH SIDGIFRGTF NNADDKVEIT 130 140 150 160 170 180 YQGCTEGFCY PPETKVLRIG DLAVSQEQIV EKTVEKNTAL LSEQDRLADG LFHSKWAIFG 190 200 210 220 230 240 FFVLGLGLAF TPCVLPMLPL LSAIVIGQQQ RPNMMRAFSL AFLYVQGMAL TYTLLGLAVA 250 260 270 280 290 300 AIGLPFQIAL QHPYVMIGLS ILFVVLALSM FGLFTIQLPN SLQNKLNTWS QKQTSGAFGG 310 320 330 340 350 360 AFAMGMIAGL VASPCTSAPL SGALLYVAQS GDLFTGAVTL YLLALGMGVP LMLITLFGNK 370 380 390 400 410 420 ILPKSGEWMN TVKQTFGFVM LALPVFLLSR ILPEVWESRL WAGLATVFFI WFALQMSKNG 430 440 450 460 470 480 FGYAIKIISF ALAMVTVQPL QNWIWQTQTT TQSAVENMPV SQVKFKQIKN TEELDRTLAE 490 500 510 520 530 540 NPHSIAMLDL YADWCVACKE FEKLTFSDPQ VQQQFQNILL LQVNMTKNSP ENKALMERFN 550 560 570 VMGLPTILFF DQQNNEIKGS RVTGFMDADA FSNWIEKLL

References

[1]

"Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains." Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D. Genome Biol. 8:RESEARCH103.1-RESEARCH103.18(2007) [PubMed: 17550610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000672 Genomic DNA. Translation: ABR00435.1.
RefSeq	YP_001292818.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5227482.
GenomeReviews	Gene locus CGSHiGG_07975 in contig CP000672_GR.
KEGG	hiq:CGSHiGG_07975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A5UI29. PVFLLSR.
Family and domain databases
HAMAP	MF_00399. [Tree]
InterPro	IPR003834. Cyt_c_assmbl_TM. IPR017936. Thioredoxin-like. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF02683. DsbD. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBD_HAEIG

Accession

Primary (citable) accession number: A5UI29

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)