A5UHQ2 (PLSB_HAEIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	CGSHiGG_07235

Organism

Haemophilus influenzae (strain PittGG) [Complete proteome] [HAMAP]

Taxonomic identifier

374931 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 810	810	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049435
Regions
	Motif	305 – 310	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A5UHQ2 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: B8204E5CB163BD26	FASTA	810	92,789
	10 20 30 40 50 60 MANFINMYRQ LLSLPLSALV KNNPIPANPI EELSLNIHQP IVYVLPYTSQ TDFVIFRRNC 70 80 90 100 110 120 LALGLPDPAE KNEINGVKLP RYVYLDEGRR IFKSKGAKDE TTTIFNKYLE LHRTSESLDV 130 140 150 160 170 180 QLIPVSVLWG RSPGQEDKSD LPNLRLLNGI QKTFAAIWFG RDTFVRFSQA VSLRYMVVEH 190 200 210 220 230 240 GSDEKIAQKL ARVAKMHFAK QRISATGPRL PNRQAMFNKL LQSEAIRRAI EDEAKSKNIS 250 260 270 280 290 300 IEKAQKEAYK ILDEIAADVS HSSLRAVDRF LRWLWNKLYS GIDVQNSNRV RKLALEGHEI 310 320 330 340 350 360 VYVPCHRSHI DYLLLSYVLY HQGLVPPHIA AGINLNFWPI GRMFRSWGAF FIRRTFKGNR 370 380 390 400 410 420 LYSAIFREYL SELFHRGYSV EYFIEGGRSR TGRLLAPKTG MMSMTLQALQ HSQTRPISIV 430 440 450 460 470 480 PVYVGYEHVL EVDTYAKELR GAAKEKENAG LVLRVIKKLR NLGQGFVNFG EPITLSNYLS 490 500 510 520 530 540 QHFPDWKEQN HEEKPQWFTP AVNNISKQVM ININKAAAVN SMNLVGTALL SSRQRALSRE 550 560 570 580 590 600 QLLEQLSSYQ QLLQNVPYST DVVLPNVTPQ AMLEHVLALD RIGVLIEKDN FGEIVRLERS 610 620 630 640 650 660 SAVLMTYYRN NIQHLFVLPS LVASIILHYE AIQKDLLLDA IRKIYPFLQG ELFLHFNEDE 670 680 690 700 710 720 LNVQIHQIIN EFARQSVINS NDNFLSINKS KVRILQLWSA GTREILQRYY ITVTILQKQP 730 740 750 760 770 780 AISRAELEKE SQLVAQRLSV LHGINAPEFF DKAVFSSFIA NLKEQRYFDE SGYTVLDKIE 790 800 810 ELASTLSHLI STEICLTVKG TIEKSEDLSS

References

[1]

"Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains." Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D. Genome Biol. 8:R103.1-R103.18(2007) [PubMed: 17550610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PittGG.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000672 Genomic DNA. Translation: ABR00308.1.
RefSeq	YP_001292691.1. NC_009567.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5UHQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5226728.
GenomeReviews	Gene locus CGSHiGG_07235 in contig CP000672_GR.
KEGG	hiq:CGSHiGG_07235.
PATRIC	20187214. VBIHaeInf102487_1459.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2937.
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_HAEIG

Accession

Primary (citable) accession number: A5UHQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program