A5UFD9 (METE_HAEIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	CGSHiGG_02260

Organism

Haemophilus influenzae (strain PittGG) [Complete proteome] [HAMAP]

Taxonomic identifier

374931 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017246
Sites
	Metal binding	642	1	Zinc By similarity
	Metal binding	644	1	Zinc By similarity
	Metal binding	727	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5UFD9 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 591EC596FAA0DC65	FASTA	756	85,193
	10 20 30 40 50 60 MTTSHILGFP RVGAKRELKF AQERYWRKEL AEQDLLDLAK ALREKNWKHQ AAANADFVAV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSQNL TLDQYFQLAR GNKDQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFQKSTA FKANPAHYVN QIREAKALGL NFKPVIVGPL TFLWLGKEKG EAFNRFDLLN 190 200 210 220 230 240 QLVPVYVEIL NALVAEGAEW IQIDEPALAL DLPAEWVEAY KSVYAELSKV NAKLLLATYF 250 260 270 280 290 300 GSVAEHAELL KALPVAGLHL DLVRAPEQLA AFEDYSKVLS AGVIEGRNIW RANLNKVLDV 310 320 330 340 350 360 LEPLKAKLGE RLWIAPSCSL LHTPFDLEVE VQLKEKNTAL YSWLSFTLQK VEELNVLKQA 370 380 390 400 410 420 LNNGRASVQA ALDASQAAAD ARATSKEIHR PEVAERLANL PKGADQRKSP FAERIVKQNA 430 440 450 460 470 480 WLNLPLLPTT NIGSFPQTTE IRHARASFKK GELSLADYEA AMKKEIEYVV RRQEELDLDV 490 500 510 520 530 540 LVHGEAERND MVEYFGELLD GFAFTKFGWV QSYGSRCVKP PVIYGDVIRP EPMTVRWSQY 550 560 570 580 590 600 AQSLTNRVMK GMLTGPVTIL QWSFVRNDIP RSTVCKQIGV ALSDEVLDLE AAGIKVIQID 610 620 630 640 650 660 EPAIREGLPL KRADWDAYLQ WAGEAFRLSS MGVQDDTQIH THMCYSEFND ILPAIAALDA 670 680 690 700 710 720 DVITIETSRS DMELLTAFAD FKYPNDIGPG VYDIHSPRVP TATEVEHLLR KALNVIPKER 730 740 750 LWVNPDCGLK TRGWTETIDQ LKVMVDVTKK LRAELA

References

[1]

"Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains." Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D. Genome Biol. 8:R103.1-R103.18(2007) [PubMed: 17550610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PittGG.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000672 Genomic DNA. Translation: ABQ99494.1.
RefSeq	YP_001291878.1. NC_009567.1.
3D structure databases
ProteinModelPortal	A5UFD9.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UFD9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5226968.
GenomeReviews	Gene locus CGSHiGG_02260 in contig CP000672_GR.
KEGG	hiq:CGSHiGG_02260.
PATRIC	20185056. VBIHaeInf102487_0453.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_HAEIG

Accession

Primary (citable) accession number: A5UFD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program