A5UE59 (ISPD_HAEIE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	CGSHiEE_08815

Organism

Haemophilus influenzae (strain PittEE) [Complete proteome] [HAMAP]

Taxonomic identifier

374930 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000022927
Sites
	Site	17	1	Transition state stabilizer By similarity
	Site	24	1	Transition state stabilizer By similarity
	Site	150	1	Positions MEP for the nucleophilic attack By similarity
	Site	206	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5UE59 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: ED172997B45E53C4	FASTA	225	24,576
	10 20 30 40 50 60 MARSIIAVLP AAGVGSRMQA DKPKQYLTLL GKTLLEHTLD VMLSYPAVSK IILAVSKDDP 70 80 90 100 110 120 YISTLSLDPK IQLVEGGTTR AESVLNGLNV IAEKNAWVLV HDAARPCLQH ADIDKLLAIE 130 140 150 160 170 180 DKQGAILAIP VTDTIKRADN QQCIVKTEDR SQLWQAMTPQ FFPVDILRDA LSTGIQQGAN 190 200 210 220 ITDEASAIEL AGFRPHLVAG RSDNLKVTRP EDLALAEFYL TRNKL

References

[1]

"Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains." Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D. Genome Biol. 8:R103.1-R103.18(2007) [PubMed: 17550610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PittEE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000671 Genomic DNA. Translation: ABQ99060.1.
RefSeq	YP_001291443.1. NC_009566.1.
3D structure databases
ProteinModelPortal	A5UE59.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UE59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5225414.
GenomeReviews	Gene locus CGSHiEE_08815 in contig CP000671_GR.
KEGG	hip:CGSHiEE_08815.
PATRIC	20279708. VBIHaeInf81350_1778.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	SKVIVVC.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	HINF374930:CGSHIEE_08815-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_HAEIE

Accession

Primary (citable) accession number: A5UE59

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program