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A5UDA6

- SYI_HAEIE

UniProt

A5UDA6 - SYI_HAEIE

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Protein
Isoleucine--tRNA ligase
Gene
ileS, CGSHiEE_07150
Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei562 – 5621Aminoacyl-adenylate By similarity
Binding sitei606 – 6061ATP By similarity
Metal bindingi904 – 9041Zinc By similarity
Metal bindingi907 – 9071Zinc By similarity
Metal bindingi924 – 9241Zinc By similarity
Metal bindingi927 – 9271Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHINF374930:GJDD-1321-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CGSHiEE_07150
OrganismiHaemophilus influenzae (strain PittEE)
Taxonomic identifieri374930 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000006555: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022072Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi374930.CGSHiEE_07150.

Structurei

3D structure databases

ProteinModelPortaliA5UDA6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotation
Add
BLAST
Motifi603 – 6075"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5UDA6-1 [UniParc]FASTAAdd to Basket

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MTVDYKNTLN LPETSFPMRG DLAKREPDML KNWYEKNLYQ KIRKASKGKK    50
SFILHDGPPY ANGNIHIGHA VNKILKDIII KSKTALGFDS PYIPGWDCHG 100
LPIELKVEGL VGKPNEKISA AEFRQKCREY ASEQVEGQKK DFIRLGVLGD 150
WDNPYLTMNF DTEANIIRTL GKVIENGHLY KGSKPVHWCL DCGSSLAEAE 200
VEYEDKVSPS IYVRFPVESA DEIEAKFSAQ GRGQGKLSAI IWTTTPWTMP 250
SNRAIAVNAD LEYNLVQLGD ERVILAAELV ESVAKAVGVE QVKILGSVKG 300
ADLELSRFHH PFYDFTVPVI LGDHVTTDGG TGLVHTAPDH GLDDFIVGKQ 350
YDLPMAGLVS NDGKFISMTE FFAGKGVFEA NPLVIEKLQE VGNLLKVEKI 400
KHSYPHCWRH KTPIIFRATP QWFIGMETQG LRQQALGEIK QVRWIPDWGQ 450
ARIEKMVENR PDWCISRQRT WGVPMTLFVH KETEELHPRT LDLLEEVAKR 500
VERAGIQAWW DLDEKELLGA DAETYRKVPD TLDVWFDSGS TYSSVVANRL 550
EFNGQNIDMY LEGSDQHRGW FMSSLMLSTA TDSKAPYKQV LTHGFTVDGQ 600
GRKMSKSIGN IVTPQEVMDK FGGDILRLWV ASTDYTGEMT VSDEILKRAA 650
DSYRRIRNTA RFLLANLNGF DPKRDLVKPE EMISLDRWAV ACALDAQHEI 700
KDAYDNYQFH TVVQRLMRFC SVEMGSFYLD IIKDRQYTTK ASSLARRSCQ 750
TALWHIAEAL VRWMAPILSF TADEIWQHLP QTESARAEFV FTEEFYQGLF 800
GLGENEKLDD AYWQQLIKVR SEVNRVLEIS RNNKEIGGGL EAEVTLYAND 850
EYRALLEQLG NELRFVLITS KVDVKSLSEK PADLADSELE GITVSVTRSN 900
AEKCPRCWHY SDKIGVNPEH PMLCPRCVEN VVGNGEVRHF A 941
Length:941
Mass (Da):106,676
Last modified:July 10, 2007 - v1
Checksum:iCCC0897EAAE0AF17
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000671 Genomic DNA. Translation: ABQ98757.1.
RefSeqiWP_005686230.1. NC_009566.1.
YP_001291140.1. NC_009566.1.

Genome annotation databases

EnsemblBacteriaiABQ98757; ABQ98757; CGSHiEE_07150.
GeneIDi5226051.
KEGGihip:CGSHiEE_07150.
PATRICi20279013. VBIHaeInf81350_1441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000671 Genomic DNA. Translation: ABQ98757.1 .
RefSeqi WP_005686230.1. NC_009566.1.
YP_001291140.1. NC_009566.1.

3D structure databases

ProteinModelPortali A5UDA6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 374930.CGSHiEE_07150.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ98757 ; ABQ98757 ; CGSHiEE_07150 .
GeneIDi 5226051.
KEGGi hip:CGSHiEE_07150.
PATRICi 20279013. VBIHaeInf81350_1441.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HINF374930:GJDD-1321-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains."
    Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D.
    Genome Biol. 8:R103.1-R103.18(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PittEE.

Entry informationi

Entry nameiSYI_HAEIE
AccessioniPrimary (citable) accession number: A5UDA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi