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A5UCW6 (DDL_HAEIE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:CGSHiEE_06350
OrganismHaemophilus influenzae (strain PittEE) [Complete proteome] [HAMAP]
Taxonomic identifier374930 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length297 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 297297D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341105

Regions

Domain95 – 294200ATP-grasp
Nucleotide binding125 – 18056ATP By similarity

Sites

Metal binding2481Magnesium or manganese 1 By similarity
Metal binding2611Magnesium or manganese 1 By similarity
Metal binding2611Magnesium or manganese 2 By similarity
Metal binding2631Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5UCW6 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: 6E657608C070D932

FASTA29732,529
        10         20         30         40         50         60 
MLLGGTSAER EVSFNSGKAV LEALLKQGYN AHPIDPKEYN VANLKKDGFN RAFNILHGRG 

        70         80         90        100        110        120 
GEDGTMQGLL EQIGLPYTGC GVMASALTMD KMRTKMLWKA FGLPVADMKV VTRETFAELD 

       130        140        150        160        170        180 
PQAVVAKLGL PLMVKPSLEG SSVGLTKVKA VEELKSAVEY ALKFDNTILI EEWLAGDELT 

       190        200        210        220        230        240 
VSVLDNQVLP AIRIVPEGEF YDYEAKYISD NTQYFCPAGL TPEREQELAI LVKRAYDAVG 

       250        260        270        280        290 
CRGWSRIDVM CDAKGNFRLV EVNTNPGMTS HSLFPKSAAT VGISFEQLVV KILELSL 

« Hide

References

[1]"Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains."
Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D.
Genome Biol. 8:R103.1-R103.18(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PittEE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000671 Genomic DNA. Translation: ABQ98617.1.
RefSeqYP_001291000.1. NC_009566.1.

3D structure databases

ProteinModelPortalA5UCW6.
SMRA5UCW6. Positions 2-296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING374930.CGSHiEE_06350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ98617; ABQ98617; CGSHiEE_06350.
GeneID5225598.
KEGGhip:CGSHiEE_06350.
PATRIC20278689. VBIHaeInf81350_1280.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycHINF374930:GJDD-1181-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_HAEIE
AccessionPrimary (citable) accession number: A5UCW6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 10, 2007
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways