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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotation

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351NADUniRule annotation
Binding sitei36 – 361NADPUniRule annotation
Active sitei156 – 1561Proton donor/acceptorUniRule annotation
Binding sitei157 – 1571SubstrateUniRule annotation
Active sitei160 – 1601Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146NAD(P)UniRule annotation
Nucleotide bindingi99 – 1013NAD(P)UniRule annotation
Nucleotide bindingi123 – 1264NAD(P)UniRule annotation

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate reductase Source: UniProtKB-EC
  2. NAD binding Source: UniProtKB-HAMAP
  3. NADPH binding Source: InterPro
  4. oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciHINF374930:GJDD-975-MONOMER.
UniPathwayiUPA00034; UER00018.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotation (EC:1.17.1.8UniRule annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:CGSHiEE_05135
OrganismiHaemophilus influenzae (strain PittEE)
Taxonomic identifieri374930 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000006555: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2702704-hydroxy-tetrahydrodipicolinate reductasePRO_1000008567Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi374930.CGSHiEE_05135.

Structurei

3D structure databases

ProteinModelPortaliA5UCB3.
SMRiA5UCB3. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 1672Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the DapB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0289.
HOGENOMiHOG000227153.
KOiK00215.
OMAiCAVYARE.
OrthoDBiEOG6SV5DS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR011770. Dihydrodipicolinate_Rdtase.
IPR023940. Dihydrodipicolinate_Rdtase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5UCB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLKIAIAGA GGRMGRQLIQ AVHSAEGVEL GAAFERKGSS LVGTDAGELA
60 70 80 90 100
GIGHLGVAVS DDLESQKDKF DLLIDFTRPE GSLEHIAFCV ANNKKMVIGT
110 120 130 140 150
TGFDQNGKAA IKAASDKIAI VFASNFSVGV NLVFKLLEKA AKVMGDYCDI
160 170 180 190 200
EVIEAHHRHK VDAPSGTALS MGEHIAKTLG RDLKTHGVFC REGITGERKR
210 220 230 240 250
DEIGFSTIRA SDVVGEHMVW FADIGERVEI SHKASSRMTF ANGAVRAGKW
260 270
LENKANGLFD MTDVLDLNNL
Length:270
Mass (Da):28,923
Last modified:July 10, 2007 - v1
Checksum:i07932E5133CFDF0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA. Translation: ABQ98414.1.
RefSeqiYP_001290797.1. NC_009566.1.

Genome annotation databases

EnsemblBacteriaiABQ98414; ABQ98414; CGSHiEE_05135.
GeneIDi5226204.
KEGGihip:CGSHiEE_05135.
PATRICi20278185. VBIHaeInf81350_1035.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA. Translation: ABQ98414.1.
RefSeqiYP_001290797.1. NC_009566.1.

3D structure databases

ProteinModelPortaliA5UCB3.
SMRiA5UCB3. Positions 3-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi374930.CGSHiEE_05135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ98414; ABQ98414; CGSHiEE_05135.
GeneIDi5226204.
KEGGihip:CGSHiEE_05135.
PATRICi20278185. VBIHaeInf81350_1035.

Phylogenomic databases

eggNOGiCOG0289.
HOGENOMiHOG000227153.
KOiK00215.
OMAiCAVYARE.
OrthoDBiEOG6SV5DS.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00018.
BioCyciHINF374930:GJDD-975-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00102. DapB.
InterProiIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR011770. Dihydrodipicolinate_Rdtase.
IPR023940. Dihydrodipicolinate_Rdtase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR20836. PTHR20836. 1 hit.
PfamiPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000161. DHPR. 1 hit.
TIGRFAMsiTIGR00036. dapB. 1 hit.
PROSITEiPS01298. DAPB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains."
    Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D.
    Genome Biol. 8:R103.1-R103.18(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PittEE.

Entry informationi

Entry nameiDAPB_HAEIE
AccessioniPrimary (citable) accession number: A5UCB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: January 7, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.