A5UBH4 (METE_HAEIE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	CGSHiEE_03520

Organism

Haemophilus influenzae (strain PittEE) [Complete proteome] [HAMAP]

Taxonomic identifier

374930 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017245
Sites
	Metal binding	642	1	Zinc By similarity
	Metal binding	644	1	Zinc By similarity
	Metal binding	727	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5UBH4 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 3F07F555F2E6165D	FASTA	756	85,151
	10 20 30 40 50 60 MTTSHILGFP RVGAKRELKF AQERYWRKEL AEQDLLDLAK ALREKNWKHQ AAANADFVAV 70 80 90 100 110 120 GDFTFYDHIL DLQVATGAIP ARFGFDSQNL TLDQYFQLAR GNKDQFAIEM TKWFDTNYHY 130 140 150 160 170 180 LVPEFQKSTA FKANPAHYVN QIREAKALGL NFKPVIVGPL TFLWLGKEKG EAFNRFDLLN 190 200 210 220 230 240 QLVPVYVEIL NALVAEGAEW IQIDEPALAL DLPAEWVEAY KSVYAELSKV NAKLLLATYF 250 260 270 280 290 300 GSVAEHAELL KALPVAGLHL DLVRAPEQLA AFEDYSKVLS AGVIEGRNIW RANLNKVLDV 310 320 330 340 350 360 LEPLKAKLGE RLWIAPSCSL LHTPFDLEVE VQLKEKNTAL YSWLSFTLQK VEELNVLKQA 370 380 390 400 410 420 LNNGRASVQA ALDASQAAAD ARATSKEIHR PEVAERLANL PKGADQRKSP FAERIVKQNA 430 440 450 460 470 480 WLNLPLLPTT NIGSFPQTTE IRHARASFKK GELSLADYEA AMKKEIEYVV RRQEELDLDV 490 500 510 520 530 540 LVHGEAERND MVEYFGELLD GFAFTKFGWV QSYGSRCVKP PVIYGDVTRP EPMTVRWSQY 550 560 570 580 590 600 AQSLTNRVMK GMLTGPVTIL QWSFVRNDIP RSTVCKQIGV ALSDEVLDLE AAGIKVIQID 610 620 630 640 650 660 EPAIREGLPL KRADWDAYLQ WAGEAFRLSS MGVQDDTQIH THMCYSEFND ILPAIAALDA 670 680 690 700 710 720 DVITIETSRS DMELLTAFAD FKYPNDIGPG VYDIHSPRVP TAAEVEHLLR KALNVIPKER 730 740 750 LWVNPDCGLK TRGWTETIDQ LKVMVDVTKK LRAELA

References

[1]

"Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains." Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D. Genome Biol. 8:R103.1-R103.18(2007) [PubMed: 17550610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PittEE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000671 Genomic DNA. Translation: ABQ98125.1.
RefSeq	YP_001290508.1. NC_009566.1.
3D structure databases
ProteinModelPortal	A5UBH4.
ModBase	Search...
Protein-protein interaction databases
STRING	A5UBH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5225774.
GenomeReviews	Gene locus CGSHiEE_03520 in contig CP000671_GR.
KEGG	hip:CGSHiEE_03520.
PATRIC	20277527. VBIHaeInf81350_0711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	HINF374930:CGSHIEE_03520-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_HAEIE

Accession

Primary (citable) accession number: A5UBH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 10, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program