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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium

Enzyme and pathway databases

BioCyciHINF374930:G1G8O-695-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:CGSHiEE_03445
OrganismiHaemophilus influenzae (strain PittEE)
Taxonomic identifieri374930 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000223441 – 863Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST863

Proteomic databases

PRIDEiA5UBF9

Structurei

3D structure databases

ProteinModelPortaliA5UBF9
SMRiA5UBF9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini446 – 568HDPROSITE-ProRule annotationAdd BLAST123
Domaini688 – 772ACT 1UniRule annotationAdd BLAST85
Domaini794 – 863ACT 2UniRule annotationAdd BLAST70

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 328UridylyltransferaseAdd BLAST328
Regioni329 – 687Uridylyl-removingAdd BLAST359

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000261778
KOiK00990
OMAiARICGYF

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 2 hits
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

A5UBF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFPLSLSSP LTPSAVKIER ENLKQFELEN FSRYSIFELV ENRSDFYDAL
60 70 80 90 100
LIQLWQEMGL SEQLGISLIA VGGYGRREMF PLSDLDFLIL VEQTPIPEIE
110 120 130 140 150
EKITQFIQFL WDCGFEVGHS VRTLQQCESE GKQDITIATN LLEARFLIGN
160 170 180 190 200
RPHFDALNEL VKRADFWSKE GFFNAKVQEQ IERYQRYHNT AYNLEPDIKY
210 220 230 240 250
SPGGLRDLHL LYWVALHHSG AQTLEDILQS GFIYPQEYQQ LQESRAFLFK
260 270 280 290 300
VRFALHLILT RYDNRLLFDR QIKVSELLGF RGEGNQAVEK MMKCFFQALH
310 320 330 340 350
RISLISNLLI QHYRENVLSS NQATVIEQLD DDFQLINQCL CLRNSLVFQE
360 370 380 390 400
KPARILDLFF YLTQYEQANI HSDTLRQLQI SLEQLPQKLC EIPEAREKFL
410 420 430 440 450
RLFNQPNSIK RAFMPMHQYG VLTAYLPQWQ AIEGLMQFDL FHIYTVDEHT
460 470 480 490 500
LRVMLKLESF LSQESAQEHP IAHRIFSQLS DRTLLYIAAL FHDIAKGRGG
510 520 530 540 550
DHAELGAKDV ANFARLHGLD RREIDTLAWL VQSHLLMSIT AQRRDIHDPE
560 570 580 590 600
VVMNFAEAVQ NQVRLDYLTC LTVADICATN GNLWNSWKRS LFASLYEFTE
610 620 630 640 650
QQFAQGMKEL LDYSEKSAEN RKLAQQILTQ DYSDIMPISI DQLWERCPED
660 670 680 690 700
YFVRNTPKQI AWHTSLLVDL VEALLVKISN RFSLGGTEVF IYCQDQPHLF
710 720 730 740 750
NKVVSTIGAK KFSIHDAQII TTQDGYVFDS FIITELNGEL VEFDRRRELE
760 770 780 790 800
QALTLALQSE KLSALSITPN RQLQHFTVQT DVRFLHENKK EHTEMELVAL
810 820 830 840 850
DKAGLLAQVS QIFSELNLNL LNAKITTVGE KAEDFFILTN QFGQALDSQQ
860
REILRNVLYR NIG
Length:863
Mass (Da):100,197
Last modified:July 10, 2007 - v1
Checksum:iFBC5A42D0FFB0B92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA Translation: ABQ98110.1

Genome annotation databases

EnsemblBacteriaiABQ98110; ABQ98110; CGSHiEE_03445
KEGGihip:CGSHiEE_03445

Similar proteinsi

Entry informationi

Entry nameiGLND_HAEIE
AccessioniPrimary (citable) accession number: A5UBF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health