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A5UBF9

- GLND_HAEIE

UniProt

A5UBF9 - GLND_HAEIE

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciHINF374930:GJDD-668-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:CGSHiEE_03445
OrganismiHaemophilus influenzae (strain PittEE)
Taxonomic identifieri374930 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000006555: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 863863Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022344Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi374930.CGSHiEE_03445.

Structurei

3D structure databases

ProteinModelPortaliA5UBF9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini446 – 579134HDUniRule annotationAdd
BLAST
Domaini688 – 77285ACT 1UniRule annotationAdd
BLAST
Domaini794 – 86370ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 328328UridylyltransferaseAdd
BLAST
Regioni329 – 687359Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5UBF9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLFPLSLSSP LTPSAVKIER ENLKQFELEN FSRYSIFELV ENRSDFYDAL
60 70 80 90 100
LIQLWQEMGL SEQLGISLIA VGGYGRREMF PLSDLDFLIL VEQTPIPEIE
110 120 130 140 150
EKITQFIQFL WDCGFEVGHS VRTLQQCESE GKQDITIATN LLEARFLIGN
160 170 180 190 200
RPHFDALNEL VKRADFWSKE GFFNAKVQEQ IERYQRYHNT AYNLEPDIKY
210 220 230 240 250
SPGGLRDLHL LYWVALHHSG AQTLEDILQS GFIYPQEYQQ LQESRAFLFK
260 270 280 290 300
VRFALHLILT RYDNRLLFDR QIKVSELLGF RGEGNQAVEK MMKCFFQALH
310 320 330 340 350
RISLISNLLI QHYRENVLSS NQATVIEQLD DDFQLINQCL CLRNSLVFQE
360 370 380 390 400
KPARILDLFF YLTQYEQANI HSDTLRQLQI SLEQLPQKLC EIPEAREKFL
410 420 430 440 450
RLFNQPNSIK RAFMPMHQYG VLTAYLPQWQ AIEGLMQFDL FHIYTVDEHT
460 470 480 490 500
LRVMLKLESF LSQESAQEHP IAHRIFSQLS DRTLLYIAAL FHDIAKGRGG
510 520 530 540 550
DHAELGAKDV ANFARLHGLD RREIDTLAWL VQSHLLMSIT AQRRDIHDPE
560 570 580 590 600
VVMNFAEAVQ NQVRLDYLTC LTVADICATN GNLWNSWKRS LFASLYEFTE
610 620 630 640 650
QQFAQGMKEL LDYSEKSAEN RKLAQQILTQ DYSDIMPISI DQLWERCPED
660 670 680 690 700
YFVRNTPKQI AWHTSLLVDL VEALLVKISN RFSLGGTEVF IYCQDQPHLF
710 720 730 740 750
NKVVSTIGAK KFSIHDAQII TTQDGYVFDS FIITELNGEL VEFDRRRELE
760 770 780 790 800
QALTLALQSE KLSALSITPN RQLQHFTVQT DVRFLHENKK EHTEMELVAL
810 820 830 840 850
DKAGLLAQVS QIFSELNLNL LNAKITTVGE KAEDFFILTN QFGQALDSQQ
860
REILRNVLYR NIG
Length:863
Mass (Da):100,197
Last modified:July 10, 2007 - v1
Checksum:iFBC5A42D0FFB0B92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA. Translation: ABQ98110.1.
RefSeqiYP_001290493.1. NC_009566.1.

Genome annotation databases

EnsemblBacteriaiABQ98110; ABQ98110; CGSHiEE_03445.
GeneIDi5225241.
KEGGihip:CGSHiEE_03445.
PATRICi20277493. VBIHaeInf81350_0696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA. Translation: ABQ98110.1 .
RefSeqi YP_001290493.1. NC_009566.1.

3D structure databases

ProteinModelPortali A5UBF9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 374930.CGSHiEE_03445.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ98110 ; ABQ98110 ; CGSHiEE_03445 .
GeneIDi 5225241.
KEGGi hip:CGSHiEE_03445.
PATRICi 20277493. VBIHaeInf81350_0696.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci HINF374930:GJDD-668-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains."
    Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D.
    Genome Biol. 8:R103.1-R103.18(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PittEE.

Entry informationi

Entry nameiGLND_HAEIE
AccessioniPrimary (citable) accession number: A5UBF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: November 26, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3