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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110ZincUniRule annotation1
Metal bindingi112ZincUniRule annotation1
Metal bindingi137ZincUniRule annotation1
Metal bindingi139ZincUniRule annotation1
Binding sitei251ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CGSHiEE_01680
OrganismiHaemophilus influenzae (strain PittEE)
Taxonomic identifieri374930 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000019071 – 480Glutamate--tRNA ligaseAdd BLAST480

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5UAL5.
SMRiA5UAL5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 31"HIGH" regionUniRule annotationAdd BLAST11
Motifi248 – 252"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252722.
KOiK01885.
OMAiQLCYMPL.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

A5UAL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLDAPFNLD PNVKVRTRFA PSPTGYLHVG GARTALYSWL YAKHNNGEFV
60 70 80 90 100
LRIEDTDLER STPEATAAII EGMEWLNLPW EHGPYYQTKR FDRYNQVIDE
110 120 130 140 150
MIEQGLAYRC YCTKEHLEEL RHTQEQNKEK PRYDRHCLHD HNHSPDEPHV
160 170 180 190 200
VRFKNPTEGS VVFDDAVRGR IEISNSELDD LIIRRTDGSP TYNFCVVVDD
210 220 230 240 250
WDMGITHVVR GEDHINNTPR QINILKAIGA PIPTYAHVSM INGDDGQKLS
260 270 280 290 300
KRHGAVSVMQ YRDDGYLPEA LINYLVRLGW GHGDQEIFSR EEMINYFELD
310 320 330 340 350
HVSKSASAFN TEKLQWLNQH YIRELPPEYV AKHLEWHYKD QGIDTSNGPA
360 370 380 390 400
LTDIVSMLAE RCKTLKEMAR SSRYFFEEFE TFDEAAAKKH FKGNAAEALT
410 420 430 440 450
KVKEKLTALS SWDLHSTHEA IEQTAAELEV GMGKVGMPLR VAVTGSGQSP
460 470 480
SMDVTLVGIG RDRVLARIQR AIDFIHAQNA
Length:480
Mass (Da):54,865
Last modified:July 10, 2007 - v1
Checksum:i540EB659106F7712
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA. Translation: ABQ97816.1.
RefSeqiWP_005686698.1. NC_009566.1.

Genome annotation databases

EnsemblBacteriaiABQ97816; ABQ97816; CGSHiEE_01680.
KEGGihip:CGSHiEE_01680.
PATRICi20276695. VBIHaeInf81350_0340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA. Translation: ABQ97816.1.
RefSeqiWP_005686698.1. NC_009566.1.

3D structure databases

ProteinModelPortaliA5UAL5.
SMRiA5UAL5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ97816; ABQ97816; CGSHiEE_01680.
KEGGihip:CGSHiEE_01680.
PATRICi20276695. VBIHaeInf81350_0340.

Phylogenomic databases

HOGENOMiHOG000252722.
KOiK01885.
OMAiQLCYMPL.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_HAEIE
AccessioniPrimary (citable) accession number: A5UAL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 10, 2007
Last modified: April 12, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.