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A5UAE5 (TRMB_HAEIE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:CGSHiEE_01300
OrganismHaemophilus influenzae (strain PittEE) [Complete proteome] [HAMAP]
Taxonomic identifier374930 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000064396

Regions

Region225 – 2284Substrate binding By similarity

Sites

Active site1521 By similarity
Binding site771S-adenosyl-L-methionine By similarity
Binding site1021S-adenosyl-L-methionine By similarity
Binding site1291S-adenosyl-L-methionine By similarity
Binding site1521S-adenosyl-L-methionine By similarity
Binding site1561Substrate By similarity
Binding site1881Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A5UAE5 [UniParc].

Last modified July 10, 2007. Version 1.
Checksum: F21A9E609CE47A05

FASTA24627,883
        10         20         30         40         50         60 
MTQTFADQKR KTVETAEFTE DGRYKRKVRS FVLRTGRLSE FQKNMMNDNW GTLGLDYQTE 

        70         80         90        100        110        120 
PFDFAKIYGN DNPVVLEIGF GMGKSLVDMA FANPDKNYLG IEVHTPGVGA CIAYAVEKGG 

       130        140        150        160        170        180 
TNLRVICHDA TEILRDSIAD GALGGLQLFF PDPWHKAKHH KRRIVQPHFV AQVVQKLAGN 

       190        200        210        220        230        240 
GFIHMATDWE NYAEQMLEVL SANTDLVNTS KNGDYIPRPD FRPLTKFEAR GHRLGHGVWD 


LYFVKK 

« Hide

References

[1]"Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains."
Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D.
Genome Biol. 8:R103.1-R103.18(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PittEE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000671 Genomic DNA. Translation: ABQ97746.1.
RefSeqYP_001290129.1. NC_009566.1.

3D structure databases

ProteinModelPortalA5UAE5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING374930.CGSHiEE_01300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ97746; ABQ97746; CGSHiEE_01300.
GeneID5226117.
KEGGhip:CGSHiEE_01300.
PATRIC20276523. VBIHaeInf81350_0260.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMALDKDHTI.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycHINF374930:GJDD-246-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HAEIE
AccessionPrimary (citable) accession number: A5UAE5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways