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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciHINF374930:GJDD-242-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:CGSHiEE_01280
OrganismiHaemophilus influenzae (strain PittEE)
Taxonomic identifieri374930 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signalUniRule annotationAdd
BLAST
Chaini34 – 827794Periplasmic nitrate reductasePRO_1000069719Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5UAE1.
SMRiA5UAE1. Positions 37-825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiFTTDEMW.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5UAE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSRRDFMK ANAAMAAATA AGLTIPVKNV VAAESEIKWD KGVCRFCGTG
60 70 80 90 100
CAVLVGTKDG RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTQPLL
110 120 130 140 150
RMTNGKFDKN GDFAPVSWDF AFKTMAEKFK EAFKKNGQNA VGMFSSGQST
160 170 180 190 200
IWEGYAKNKL WKAGFRSNNV DPNARHCMAS AAVAFMRTFG MDEPMGCYDD
210 220 230 240 250
IEQADAFVLW GSNMAEMHPI LWSRITDRRI SNPDVRVTVL STYEHRSFEL
260 270 280 290 300
ADHGLIFTPQ TDLAIMNYII NYLIQNNAIN WDFVNKHTKF KRGETNIGYG
310 320 330 340 350
LRPEHPLEKD TNRKTAGKMH DSSFEELKQL VSEYTVEKVS QMSGLDKVQL
360 370 380 390 400
ETLAKLYADP TKKVVSYWTM GFNQHTRGVW VNQLIYNIHL LTGKISIPGC
410 420 430 440 450
GPFSLTGQPS ACGTAREVGS FPHRLPADLV VTNPKHREIA ERIWKLPKGT
460 470 480 490 500
VSEKVGLHTI AQDRAMNDGK MNVLWQMCNN NMQAGPNINQ ERLPGWRKEG
510 520 530 540 550
NFVIVSDPYP TVSALSADLI LPTAMWVEKE GAYGNAERRT QFWRQQVKAP
560 570 580 590 600
GEAKSDLWQL MEFAKYFTTD EMWTEELLAQ MPEYRGKTLY EVLFKNGQVD
610 620 630 640 650
KFPLSELAEG QLNDESEYFG YYVHKGLFEE YAEFGRGHGH DLAPFDMYHK
660 670 680 690 700
ARGLRWPVVE GKETLWRYRE GYDPYVKEGE GVAFYGYPDK KAIILAVPYE
710 720 730 740 750
PPAESPDNEY DLWLSTGRVL EHWHTGTMTR RVPELHRAFP NNLVWMHPLD
760 770 780 790 800
AQARGLRHGD KIKISSRRGE MISYLDTRGR NKPPRGLVFT TFFDAGQLAN
810 820
NLTLDATDPI SKETDFKKCA VKVEKAA
Length:827
Mass (Da):93,713
Last modified:July 10, 2007 - v1
Checksum:i91B856383FCFA477
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA. Translation: ABQ97742.1.
RefSeqiWP_005686782.1. NC_009566.1.
YP_001290125.1. NC_009566.1.

Genome annotation databases

EnsemblBacteriaiABQ97742; ABQ97742; CGSHiEE_01280.
KEGGihip:CGSHiEE_01280.
PATRICi20276515. VBIHaeInf81350_0256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000671 Genomic DNA. Translation: ABQ97742.1.
RefSeqiWP_005686782.1. NC_009566.1.
YP_001290125.1. NC_009566.1.

3D structure databases

ProteinModelPortaliA5UAE1.
SMRiA5UAE1. Positions 37-825.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ97742; ABQ97742; CGSHiEE_01280.
KEGGihip:CGSHiEE_01280.
PATRICi20276515. VBIHaeInf81350_0256.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiFTTDEMW.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciHINF374930:GJDD-242-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains."
    Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D.
    Genome Biol. 8:R103.1-R103.18(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PittEE.

Entry informationi

Entry nameiNAPA_HAEIE
AccessioniPrimary (citable) accession number: A5UAE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 10, 2007
Last modified: June 24, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.