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A5UAE1

- NAPA_HAEIE

UniProt

A5UAE1 - NAPA_HAEIE

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi79 – 791Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciHINF374930:GJDD-242-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:CGSHiEE_01280
    OrganismiHaemophilus influenzae (strain PittEE)
    Taxonomic identifieri374930 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000006555: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Tat-type signalUniRule annotationAdd
    BLAST
    Chaini34 – 827794Periplasmic nitrate reductasePRO_1000069719Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi374930.CGSHiEE_01280.

    Structurei

    3D structure databases

    ProteinModelPortaliA5UAE1.
    SMRiA5UAE1. Positions 37-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 93574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiAGKMHDS.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A5UAE1-1 [UniParc]FASTAAdd to Basket

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    MNLSRRDFMK ANAAMAAATA AGLTIPVKNV VAAESEIKWD KGVCRFCGTG    50
    CAVLVGTKDG RVVASQGDPD AEVNRGLNCI KGYFLPKIMY GKDRLTQPLL 100
    RMTNGKFDKN GDFAPVSWDF AFKTMAEKFK EAFKKNGQNA VGMFSSGQST 150
    IWEGYAKNKL WKAGFRSNNV DPNARHCMAS AAVAFMRTFG MDEPMGCYDD 200
    IEQADAFVLW GSNMAEMHPI LWSRITDRRI SNPDVRVTVL STYEHRSFEL 250
    ADHGLIFTPQ TDLAIMNYII NYLIQNNAIN WDFVNKHTKF KRGETNIGYG 300
    LRPEHPLEKD TNRKTAGKMH DSSFEELKQL VSEYTVEKVS QMSGLDKVQL 350
    ETLAKLYADP TKKVVSYWTM GFNQHTRGVW VNQLIYNIHL LTGKISIPGC 400
    GPFSLTGQPS ACGTAREVGS FPHRLPADLV VTNPKHREIA ERIWKLPKGT 450
    VSEKVGLHTI AQDRAMNDGK MNVLWQMCNN NMQAGPNINQ ERLPGWRKEG 500
    NFVIVSDPYP TVSALSADLI LPTAMWVEKE GAYGNAERRT QFWRQQVKAP 550
    GEAKSDLWQL MEFAKYFTTD EMWTEELLAQ MPEYRGKTLY EVLFKNGQVD 600
    KFPLSELAEG QLNDESEYFG YYVHKGLFEE YAEFGRGHGH DLAPFDMYHK 650
    ARGLRWPVVE GKETLWRYRE GYDPYVKEGE GVAFYGYPDK KAIILAVPYE 700
    PPAESPDNEY DLWLSTGRVL EHWHTGTMTR RVPELHRAFP NNLVWMHPLD 750
    AQARGLRHGD KIKISSRRGE MISYLDTRGR NKPPRGLVFT TFFDAGQLAN 800
    NLTLDATDPI SKETDFKKCA VKVEKAA 827
    Length:827
    Mass (Da):93,713
    Last modified:July 10, 2007 - v1
    Checksum:i91B856383FCFA477
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000671 Genomic DNA. Translation: ABQ97742.1.
    RefSeqiWP_005686782.1. NC_009566.1.
    YP_001290125.1. NC_009566.1.

    Genome annotation databases

    EnsemblBacteriaiABQ97742; ABQ97742; CGSHiEE_01280.
    GeneIDi5225788.
    KEGGihip:CGSHiEE_01280.
    PATRICi20276515. VBIHaeInf81350_0256.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000671 Genomic DNA. Translation: ABQ97742.1 .
    RefSeqi WP_005686782.1. NC_009566.1.
    YP_001290125.1. NC_009566.1.

    3D structure databases

    ProteinModelPortali A5UAE1.
    SMRi A5UAE1. Positions 37-825.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 374930.CGSHiEE_01280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ97742 ; ABQ97742 ; CGSHiEE_01280 .
    GeneIDi 5225788.
    KEGGi hip:CGSHiEE_01280.
    PATRICi 20276515. VBIHaeInf81350_0256.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi AGKMHDS.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci HINF374930:GJDD-242-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains."
      Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D.
      Genome Biol. 8:R103.1-R103.18(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PittEE.

    Entry informationi

    Entry nameiNAPA_HAEIE
    AccessioniPrimary (citable) accession number: A5UAE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3