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A5UAC2

- RLMN_HAEIE

UniProt

A5UAC2 - RLMN_HAEIE

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Protein

Dual-specificity RNA methyltransferase RlmN

Gene
rlmN, CGSHiEE_01185
Organism
Haemophilus influenzae (strain PittEE)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity By similarity.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA.UniRule annotation
2 S-adenosyl-L-methionine + adenine(37) in tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101Proton acceptor Reviewed prediction
Metal bindingi130 – 1301Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi134 – 1341Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi137 – 1371Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Binding sitei216 – 2161S-adenosyl-L-methionine By similarity
Binding sitei317 – 3171S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
Active sitei360 – 3601S-methylcysteine intermediate By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. rRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
  4. rRNA binding Source: UniProtKB-HAMAP
  5. tRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
  6. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. rRNA base methylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHINF374930:GJDD-223-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual-specificity RNA methyltransferase RlmN (EC:2.1.1.-, EC:2.1.1.192)
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferase
23S rRNA m2A2503 methyltransferase
Ribosomal RNA large subunit methyltransferase N
tRNA (adenine(37)-C(2))-methyltransferase
tRNA m2A37 methyltransferase
Gene namesi
Name:rlmN
Ordered Locus Names:CGSHiEE_01185
OrganismiHaemophilus influenzae (strain PittEE)
Taxonomic identifieri374930 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000006555: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Dual-specificity RNA methyltransferase RlmNUniRule annotationPRO_0000350203Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi123 ↔ 360(transient) By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi374930.CGSHiEE_01185.

Structurei

3D structure databases

ProteinModelPortaliA5UAC2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 1852S-adenosyl-L-methionine binding By similarity
Regioni238 – 2403S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0820.
HOGENOMiHOG000217992.
KOiK06941.
OrthoDBiEOG6DJZ2N.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01849. RNA_methyltr_RlmN.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR30544. PTHR30544. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF006004. CHP00048. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00048. TIGR00048. 1 hit.

Sequencei

Sequence statusi: Complete.

A5UAC2-1 [UniParc]FASTAAdd to Basket

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MCNNEAKMSE LLSVQSDAPA KKINLMDLTR QQMREFFKEL GEKPFRADQL    50
VKWIYHFGED NFDNMTNINK KLREKLKAVA EIKAPEVAVE QRSADGTIKW 100
AMQVGEQQVE TVYIPEADRA TLCVSSQVGC ALACTFCSTA QQGFNRNLTV 150
SEIIGQVWRA SKIIGNFGVT GVRPITNVVM MGMGEPLLNV ANVVPAMEIM 200
LDDFAYGLSK RRVTLSTSGV VPALDNLSKM IDVALAISLH APNDELRDEI 250
VPINKKYNIK TLIDSVNRYL TVSNANHGKV TIEYVMLDHV NDGVEHAHQL 300
ADVLKNTPCK INLIPWNPFP EAPYAKSSNT RIDRFQKTLM EYDFTVIIRK 350
TRGDDIDAAC GQLAGDVIDR TKRTAMKRQF GQNIGVTEVN 390
Length:390
Mass (Da):43,570
Last modified:September 2, 2008 - v2
Checksum:i85E174CE9CF12465
GO

Sequence cautioni

The sequence ABQ97723.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000671 Genomic DNA. Translation: ABQ97723.1. Different initiation.
RefSeqiYP_001290106.1. NC_009566.1.

Genome annotation databases

EnsemblBacteriaiABQ97723; ABQ97723; CGSHiEE_01185.
GeneIDi5224754.
KEGGihip:CGSHiEE_01185.
PATRICi20276473. VBIHaeInf81350_0235.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000671 Genomic DNA. Translation: ABQ97723.1 . Different initiation.
RefSeqi YP_001290106.1. NC_009566.1.

3D structure databases

ProteinModelPortali A5UAC2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 374930.CGSHiEE_01185.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ97723 ; ABQ97723 ; CGSHiEE_01185 .
GeneIDi 5224754.
KEGGi hip:CGSHiEE_01185.
PATRICi 20276473. VBIHaeInf81350_0235.

Phylogenomic databases

eggNOGi COG0820.
HOGENOMi HOG000217992.
KOi K06941.
OrthoDBi EOG6DJZ2N.

Enzyme and pathway databases

BioCyci HINF374930:GJDD-223-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01849. RNA_methyltr_RlmN.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR30544. PTHR30544. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF006004. CHP00048. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00048. TIGR00048. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains."
    Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C., Ehrlich G.D.
    Genome Biol. 8:R103.1-R103.18(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PittEE.

Entry informationi

Entry nameiRLMN_HAEIE
AccessioniPrimary (citable) accession number: A5UAC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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